Facultad de Química, Bioquímica y Farmacia, Instituto de Química de San Luis, INQUISAL (UNSL-CONICET), Universidad Nacional de San Luis, Chacabuco 917, San Luis D5700BWS, Argentina.
GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government PTS, Granada, Avenida de la Ilustración, 114, 18016 Granada, Spain.
Biosensors (Basel). 2023 Mar 16;13(3):390. doi: 10.3390/bios13030390.
Prostate cancer is a disease with a high incidence and mortality rate in men worldwide. Serum prostate-specific antigens (PSA) are the main circulating biomarker for this disease in clinical practices. In this work, we present a portable and reusable microfluidic device for PSA quantification. This device comprises a polymethyl methacrylate microfluidic platform coupled with electrochemical detection. The platinum working microelectrode was positioned in the outflow region of the microchannel and was modified with carbon nanofibers (CNF)-decorated gold nanoporous (GNP) structures by the dynamic hydrogen bubble template method, through the simultaneous electrodeposition of metal precursors in the presence of CNF. CNF/GNP structures exhibit attractive properties, such as a large surface to volume ratio, which increases the antibody's immobilization capacity and the electroactive area. CNFs/GNP structures were characterized by scanning electron microscopy, energy dispersive spectrometry, and cyclic voltammetry. Anti-PSA antibodies and HRP were employed for the immune-electrochemical reaction. The detection limit for the device was 5 pg mL, with a linear range from 0.01 to 50 ng mL. The coefficients of variation within and between assays were lower than 4.40%, and 6.15%, respectively. Additionally, its clinical performance was tested in serum from 30 prostate cancer patients. This novel device was a sensitive, selective, portable, and reusable tool for the serological diagnosis and monitoring of prostate cancer.
前列腺癌是一种在全球男性中发病率和死亡率都很高的疾病。血清前列腺特异性抗原（PSA）是临床实践中用于诊断这种疾病的主要循环生物标志物。在这项工作中，我们提出了一种用于 PSA 定量的便携式、可重复使用的微流控装置。该装置由聚甲基丙烯酸甲酯微流控平台与电化学检测相结合组成。铂工作微电极位于微通道的流出区，并通过动态氢气泡模板法用碳纳米纤维（CNF）修饰的金纳米多孔（GNP）结构进行修饰，该方法通过在存在 CNF 的情况下同时电沉积金属前体。CNF/GNP 结构具有吸引人的特性，例如大的表面积与体积比，这增加了抗体的固定容量和电活性面积。通过扫描电子显微镜、能谱和循环伏安法对 CNF/GNP 结构进行了表征。抗 PSA 抗体和 HRP 用于免疫电化学反应。该装置的检测限为 5 pg mL，线性范围为 0.01 至 50 ng mL。分析内和分析间的变异系数均低于 4.40%和 6.15%。此外，还在 30 名前列腺癌患者的血清中测试了其临床性能。这种新型装置是一种用于前列腺癌血清学诊断和监测的灵敏、选择性、便携式和可重复使用的工具。
