Department of Pharmaceutical Sciences and Center for Biomolecular Sciences, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60607, USA.
Department of Pharmaceutical Sciences and Center for Biomolecular Sciences, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60607, USA.
Curr Opin Biotechnol. 2022 Oct;77:102782. doi: 10.1016/j.copbio.2022.102782. Epub 2022 Aug 29.
Bacterial natural products (NPs) retain high value in discovery efforts for applications in medicine and agriculture. Burkholderia β-Proteobacteria are a promising source of NPs. In this review, we summarize the recently developed genetic manipulation techniques used to access silent/cryptic biosynthetic gene clusters from Burkholderia native producers. We also discuss the development of Burkholderia bacteria as heterologous hosts and the application of Burkholderia in industrial-scale production of NPs. Genetic engineering and fermentation media optimization have enabled the industrial-scale production of at least two Burkholderia NPs. The biotechnology approaches discussed here will continue to facilitate the discovery and development of NPs from Burkholderia.
细菌天然产物（NPs）在医学和农业应用的发现工作中具有很高的价值。β-变形菌中的伯克霍尔德菌是 NPs 的一个很有前途的来源。在这篇综述中，我们总结了最近开发的遗传操作技术，用于从伯克霍尔德菌天然产生菌中获得沉默/隐匿生物合成基因簇。我们还讨论了将伯克霍尔德菌作为异源宿主的发展以及在 NPs 的工业规模生产中的应用。遗传工程和发酵培养基的优化使至少两种 Burkholderia NPs 的工业规模生产成为可能。这里讨论的生物技术方法将继续促进 Burkholderia 中 NPs 的发现和开发。
