Child and Adolescent Health, School of Public Health, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang, 110122, PR China; Liaoning Key Laboratory of Obesity and Glucose/Lipid Associated Metabolic Diseases, Shenyang, 110122, China.
Child and Adolescent Health, School of Public Health, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang, 110122, PR China; Liaoning Key Laboratory of Obesity and Glucose/Lipid Associated Metabolic Diseases, Shenyang, 110122, China.
Food Chem Toxicol. 2022 Nov;169:113394. doi: 10.1016/j.fct.2022.113394. Epub 2022 Aug 30.
Increased fructose intake is a global issue, especially in mothers. Maternal fructose exposure during gestation and lactation can affect learning and memory in offspring; however, the detailed mechanism is still unknown. The hippocampus is a mind locale liable for learning and memory. Here, we established a maternal high-fructose diet model by administering 13% and 40% fructose water, applied the Morris Water Maze test on postnatal day 60 offspring, and performed full-length RNA sequencing using the Oxford Nanopore Technologies platform to explore the changes in gene expression in the hippocampus. The results showed that learning and memory in offspring were negatively affected. Compared with the control group, 369 differentially expressed transcripts (DETs) were identified in the 13% fructose group, and 501 DETs were identified in the 40% fructose group. Gene Ontology enriched term and Kyoto Encyclopedia of Genes and Genomes enriched pathway analyses identified several terms and pathways related to brain development and cognitive function. Furthermore, we confirmed that the Wnt/β-catenin signaling pathway was down-regulated and neuron degeneration was enhanced. In summary, our results indicate that maternal fructose exposure during gestation and lactation can impair learning and memory in offspring and affect brain function at the transcriptome level.
果糖摄入量增加是一个全球性问题，尤其是在母亲中。母体在妊娠和哺乳期摄入果糖会影响后代的学习和记忆；然而，其详细机制尚不清楚。海马体是一个负责学习和记忆的脑区。在这里，我们通过给予 13%和 40%的果糖水建立了母体高果糖饮食模型，在产后第 60 天对后代进行了 Morris 水迷宫测试，并使用 Oxford Nanopore Technologies 平台进行全长 RNA 测序，以探索海马体中基因表达的变化。结果表明，后代的学习和记忆受到负面影响。与对照组相比，13%果糖组中鉴定出 369 个差异表达转录本（DETs），40%果糖组中鉴定出 501 个 DETs。基因本体富集术语和京都基因与基因组百科全书富集途径分析确定了几个与脑发育和认知功能相关的术语和途径。此外，我们证实 Wnt/β-连环蛋白信号通路下调，神经元退化增强。总之，我们的结果表明，母体在妊娠和哺乳期摄入果糖会损害后代的学习和记忆，并在转录组水平上影响大脑功能。
