Child and Adolescent Health, School of Public Health, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang 110122, China.
Int J Mol Sci. 2023 Feb 24;24(5):4460. doi: 10.3390/ijms24054460.
Increased fructose intake is an international issue. A maternal high-fructose diet during gestation and lactation could affect nervous system development in offspring. Long non-coding RNA (lncRNA) plays an important role in brain biology. However, the mechanism whereby maternal high-fructose diets influence offspring brain development by affecting lncRNAs is still unclear. Here, we administered 13% and 40% fructose water to establish a maternal high-fructose diet model during gestation and lactation. To determine lncRNAs and their target genes, full-length RNA sequencing was performed using the Oxford Nanopore Technologies platform, and 882 lncRNAs were identified. Moreover, the 13% fructose group and the 40% fructose group had differentially expressed lncRNA genes compared with the control group. Enrichment analyses and co-expression analyses were performed to investigate the changes in biological function. Furthermore, enrichment analyses, behavioral science experiments, and molecular biology experiments all indicated that the fructose group offspring showed anxiety-like behaviors. In summary, this study provides insight into the molecular mechanisms underlying maternal high-fructose diet-induced lncRNA expression and co-expression of lncRNA and mRNA.
果糖摄入增加是一个国际性问题。母体在妊娠和哺乳期的高果糖饮食可能会影响后代的神经系统发育。长链非编码 RNA（lncRNA）在大脑生物学中发挥着重要作用。然而，母体高果糖饮食通过影响 lncRNA 影响后代大脑发育的机制尚不清楚。在这里，我们通过给母体喂食 13%和 40%的果糖水，建立了妊娠和哺乳期的母体高果糖饮食模型。为了确定 lncRNA 及其靶基因，我们使用牛津纳米孔技术平台进行了全长 RNA 测序，鉴定出了 882 个 lncRNA。此外，与对照组相比，13%果糖组和 40%果糖组的 lncRNA 基因表达存在差异。进行了富集分析和共表达分析，以研究生物学功能的变化。此外，富集分析、行为科学实验和分子生物学实验均表明，果糖组后代表现出焦虑样行为。总之，本研究为母体高果糖饮食诱导的 lncRNA 表达以及 lncRNA 和 mRNA 的共表达的分子机制提供了新的见解。
