Ghosh Ajit, Islam Md Sifatul, Alam Nazmir Binta, Mustafiz Ananda, Islam Tahmina
Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet, 3114 Bangladesh.
Plant Molecular Biology Laboratory, Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi, India.
Physiol Mol Biol Plants. 2022 Jul;28(7):1375-1390. doi: 10.1007/s12298-022-01220-5. Epub 2022 Aug 15.
Homoeostasis of glutathione (GSH) is crucial for plant survival and adaptability against stress. Despite the presence of complete and rice genome sequence, the comprehensive analysis of the GSH metabolizing genes is still missing. This research concentrated on the comprehensive understanding of GSH metabolizing genes in two model plants- and rice in terms of their subcellular localization, exon-intron distribution, protein domain structure, and transcript abundance. Expression profiling using the microarray data provided significant evidence of their participation in response to various abiotic stress conditions. Besides, some of these GSH metabolizing genes revealed their expression alteration in several developmental changes and tissue diversification. The presence of various stress-specific cis-regulatory elements in the promoter region of GSH metabolizing genes could be directly correlated with their stress-specific transcript alteration. Moreover, the application of exogenous GSH significantly downregulated GSH synthesizing genes and upregulated GSH metabolizing genes in with few exceptions indicating a product-dependent regulation of GSH metabolizing genes. Interestingly, validation of rice GSH metabolizing genes in response to drought and salinity showed an almost similar pattern of expression in quantitative real-time as observed by microarray data. Altogether, GSH metabolizing members are a promising and underutilized genetic source for plant improvement that could be used to enhance stress tolerance in plants.
The online version contains supplementary material available at 10.1007/s12298-022-01220-5.
谷胱甘肽（GSH）的稳态对于植物的存活以及应对胁迫的适应能力至关重要。尽管已有完整的拟南芥和水稻基因组序列，但对谷胱甘肽代谢相关基因的全面分析仍缺失。本研究着重全面了解两种模式植物——拟南芥和水稻中谷胱甘肽代谢相关基因的亚细胞定位、外显子 - 内含子分布、蛋白质结构域结构以及转录本丰度。利用微阵列数据进行的表达谱分析提供了它们参与应对各种非生物胁迫条件的重要证据。此外，其中一些谷胱甘肽代谢相关基因在多个发育变化和组织分化过程中显示出表达变化。谷胱甘肽代谢相关基因启动子区域存在的各种胁迫特异性顺式调控元件可能与其胁迫特异性转录本变化直接相关。而且，外源谷胱甘肽的应用显著下调了拟南芥中谷胱甘肽合成基因的表达，并上调了谷胱甘肽代谢基因的表达，少数情况除外，这表明谷胱甘肽代谢相关基因存在产物依赖性调控。有趣的是，水稻谷胱甘肽代谢相关基因对干旱和盐胁迫响应的验证表明，在定量实时分析中其表达模式与微阵列数据观察到的几乎相似。总之，谷胱甘肽代谢成员是植物改良中一个有前景但未充分利用的遗传资源，可用于增强植物的胁迫耐受性。
在线版本包含可在10.1007/s12298 - 022 - 01220 - 5获取的补充材料。
