School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Affiliated Hospital of Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China.
Contrast Media Mol Imaging. 2022 Aug 18;2022:3517020. doi: 10.1155/2022/3517020. eCollection 2022.
Pure plant extract luteolin has been demonstrated to possess numerous biological and immunological effects. However, how luteolin affects mice alveolar macrophages' self-renewal and polarization closely related to inflammatory and immunomodulatory is still unknown. In our study, the transcriptomic analysis showed that several self-renewal-related pathways in luteolin-pretreated alveolar macrophages were inhibited compared to the granulocyte-macrophage colony-stimulating factor (GM-CSF)-treated group. Ki-67 staining and EdU assay indicated that luteolin inhibited GM-CSF-induced alveolar macrophage proliferation. Moreover, GM-CSF-induced expressions of c-Myc and KLF4 were significantly suppressed by luteolin at transcriptional and protein levels. Besides, we found that luteolin promoted M1 macrophage polarization induced by LPS plus IFN-. At the same time, it inhibited M2 macrophage polarization induced by IL-4 in both alveolar and bone marrow-derived macrophages by detecting macrophage polarization-related gene expressions at mRNA and protein levels. We found that luteolin inhibited self-renewal and altered the polarization of primary alveolar macrophages. Taken together, our data will aid in a better understanding of the immunomodulatory effects of luteolin on the primary alveolar macrophages.
纯植物提取物木犀草素已被证明具有许多生物学和免疫学作用。然而，木犀草素如何影响与炎症和免疫调节密切相关的小鼠肺泡巨噬细胞的自我更新和极化仍不清楚。在我们的研究中，转录组分析表明，与粒细胞-巨噬细胞集落刺激因子（GM-CSF）处理组相比，木犀草素预处理的肺泡巨噬细胞中几个与自我更新相关的途径受到抑制。Ki-67 染色和 EdU 检测表明，木犀草素抑制 GM-CSF 诱导的肺泡巨噬细胞增殖。此外，木犀草素在转录和蛋白水平上显著抑制 GM-CSF 诱导的 c-Myc 和 KLF4 表达。此外，我们发现木犀草素通过检测巨噬细胞极化相关基因在 mRNA 和蛋白水平上的表达，促进 LPS 加 IFN-诱导的 M1 巨噬细胞极化，同时抑制 IL-4 诱导的 M2 巨噬细胞极化。在肺泡和骨髓来源的巨噬细胞中。我们发现木犀草素抑制了原代肺泡巨噬细胞的自我更新并改变了其极化。总之，我们的数据将有助于更好地理解木犀草素对原代肺泡巨噬细胞的免疫调节作用。
