Zhu Zhen-Hua, Yin Xu-Yuan, Xu Tu-Sun, Tao Wei-Wei, Yao Guang-Da, Wang Pei-Jie, Qi Qi, Jia Qiu-Fang, Wang Jing, Zhu Yue, Hui Li
Research Center of Biological Psychiatry, Suzhou Guangji Hospital, Medical College of Soochow University, Suzhou, China.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
Front Pharmacol. 2022 Aug 16;13:855964. doi: 10.3389/fphar.2022.855964. eCollection 2022.
oligosaccharides (MOs) are natural herbal extracts that have been shown to exert antidepressant effects. However, the mechanism of this effect remains unclear. Here, we explored the mechanism by which MOs improved experimental depression. Using a chronic mild stress (CMS) murine model, we examined whether MOs could protect against depressive-like behaviour. Lipopolysaccharide (LPS)- and ATP-treated BV2 cells were used to examine the potential mechanism by which MOs mediate the inflammatory response. We found that MOs prevented the CMS-induced reduction in the sucrose preference ratio in the sucrose preference test (SPT) and shortened the immobility durations in both the tail suspension test (TST) and forced swim test (FST). We also noticed that MOs suppressed inflammatory effects by deactivating the MyD88/PI3K pathway E2F2 in CMS mice or LPS- and ATP-stimulated BV2 cells. Furthermore, overexpression of E2F2 blunted the beneficial effects of MOs . Collectively, these data showed that MOs exerted antidepressant effects in CMS mice by targeting E2F2-mediated MyD88/PI3K signalling pathway.
寡糖（MOs）是已被证明具有抗抑郁作用的天然草药提取物。然而，这种作用的机制仍不清楚。在此，我们探讨了MOs改善实验性抑郁的机制。使用慢性轻度应激（CMS）小鼠模型，我们研究了MOs是否能预防类似抑郁的行为。用脂多糖（LPS）和ATP处理的BV2细胞来研究MOs介导炎症反应的潜在机制。我们发现，MOs在蔗糖偏好试验（SPT）中防止了CMS诱导的蔗糖偏好率降低，并缩短了尾悬试验（TST）和强迫游泳试验（FST）中的不动时间。我们还注意到，MOs通过使CMS小鼠或LPS和ATP刺激的BV2细胞中的MyD88/PI3K途径E2F2失活来抑制炎症作用。此外，E2F2的过表达减弱了MOs的有益作用。总的来说，这些数据表明，MOs通过靶向E2F2介导的MyD88/PI3K信号通路在CMS小鼠中发挥抗抑郁作用。
