State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
Faculty of Medicine, Macau University of Science and Technology, Taipa, Macau 999078, China.
Oxid Med Cell Longev. 2022 Aug 23;2022:5025237. doi: 10.1155/2022/5025237. eCollection 2022.
Alcoholic liver disease (ALD) is a major public health problem worldwide, which needs to be effective prevention. Ginsenoside Rg1 (GRg1), a bioactive ingredient extracted from ginseng, has benefit effects on health. In this study, 11 potential targets of GRg1 against ALD were firstly obtained by network pharmacology. KEGG pathway enrichment showed that GRg1-target-ALD was closely related to Toll-like receptor (TLR) and nuclear factor-kappa B (NF-B) signaling pathways. In addition, GRg1 decreased antioxidant levels and increased oxidative levels in alcohol-treated mice, which alleviated oxidative stress-induced hepatic damage. GRg1 enhanced intestinal barrier function upregulating the levels of tight junction protein and immunoglobulin A. GRg1 also reduced alcohol-induced inflammation by suppressing TLR4/NF-B pathway, which was consistent with the prediction of network targets. Moreover, GRg1 altered GM population, and Verrucomicrobia, Bacteroidetes, , , , and played positive association with intestinal barrier indicators and negative correlation with hepatic inflammation biomarkers. The results suggest that GRg1 administration might be a promising strategy for protection of alcohol-induced liver damage.
酒精性肝病（ALD）是全球范围内的一个主要公共卫生问题，需要采取有效的预防措施。人参皂苷 Rg1（GRg1）是从人参中提取的一种具有生物活性的成分，对健康有益。在这项研究中，首先通过网络药理学获得了 11 个针对 ALD 的 GRg1 潜在靶标。KEGG 通路富集表明，GRg1-靶标-ALD 与 Toll 样受体（TLR）和核因子-κB（NF-κB）信号通路密切相关。此外，GRg1 降低了酒精处理小鼠的抗氧化水平，增加了氧化水平，从而减轻了氧化应激引起的肝损伤。GRg1 通过抑制 TLR4/NF-κB 通路增强肠道屏障功能，上调紧密连接蛋白和免疫球蛋白 A 的水平。GRg1 还通过抑制 TLR4/NF-κB 通路抑制酒精诱导的炎症，与网络靶标的预测一致。此外，GRg1 改变了 GM 群体，而厚壁菌门、拟杆菌门、变形菌门、放线菌门、梭杆菌门和螺旋体门与肠道屏障指标呈正相关，与肝炎症生物标志物呈负相关。这些结果表明，GRg1 给药可能是预防酒精性肝损伤的一种有前途的策略。
