Al-Ramahi Nadia, Al-Najjar Mohammad A A, Jabaley Abdalluh, Al-Qudah Rajaa, Basheti Iman
Pharmaceutical Sciences Program. Faculty of Pharmacy, Applied Science, Private University, Jordan.
Department of Pharmaceutical Sciences and Pharmaceutics, Applied Science, Private University, Jordan.
Saudi J Biol Sci. 2022 Oct;29(10):103406. doi: 10.1016/j.sjbs.2022.103406. Epub 2022 Aug 10.
In this work, variation in microbiota in the lower respiratory tract (LRT) among asthmatic and non-asthmatic subjects is identified. All participants (27 asthmatic patients and 27 non-asthmatic subjects) were asked to expectorate a sputum sample in special sterile tubes after rinsing the mouth with a sterilizing solution. The expectorated sputum specimen was immediately homogenized and stored in the deep freezer for DNA extraction for microbial gene sequencing and sequence analyses. For sequencing the V4 region of the 16S rRNA gene was sequenced using Illumina MiSeq, followed by an analysis of alpha and beta diversity. It was found that asthmatic patients had greater bacterial diversity than non-asthmatic subjects. Bacteria associated to the phyla (Bacteroidetes, Proteobacteria, and Firmicutes) accounted for 90 % of all sequences. The relative abundance of Proteobacteria in the asthmatic patients was higher than that of non-asthmatic (30 % vs 17 %; P-value = 0.044), along with a high abundance of the pathogen . In contrast, Firmicutes (41 %) and Bacteroidetes (31 %) showed higher relative abundances in the non-asthmatic subjects. No significant link was found between the type of asthma drug or the method of drug usage (orally or via inhalation) and the respiratory microbiota. Therefore, the variations in LRS microbiota are not caused by the drugs taken by the asthmatic patients, rather they might be connected to the etiology of asthma. Since the asthmatic patients had higher proportions of , these organisms could be a causative factor in the pathophysiology of asthma.
在这项研究中，确定了哮喘患者和非哮喘患者下呼吸道（LRT）微生物群的差异。所有参与者（27名哮喘患者和27名非哮喘受试者）在用消毒溶液漱口后，被要求在特殊的无菌试管中咳出痰样本。咳出的痰标本立即匀浆，并储存在深冻冰箱中用于提取DNA，以进行微生物基因测序和序列分析。为了进行测序，使用Illumina MiSeq对16S rRNA基因的V4区域进行测序，随后分析α和β多样性。结果发现，哮喘患者的细菌多样性高于非哮喘受试者。与拟杆菌门、变形菌门和厚壁菌门相关的细菌占所有序列的90%。哮喘患者中变形菌门的相对丰度高于非哮喘患者（30%对17%；P值=0.044），同时病原体丰度也很高。相比之下，厚壁菌门（41%）和拟杆菌门（31%）在非哮喘受试者中显示出更高的相对丰度。未发现哮喘药物类型或用药方法（口服或吸入）与呼吸道微生物群之间存在显著联系。因此，LRS微生物群的差异不是由哮喘患者服用的药物引起的，而是可能与哮喘的病因有关。由于哮喘患者中[此处原文缺失相关内容]的比例较高，这些微生物可能是哮喘病理生理学中的一个致病因素。
