Department of Stomatology, The Second Xiangya Hospital of Central South University, Changsha, Hunan, 410011, China.
National Clinical Research Center for Metabolic Diseases, Hunan Provincial Key Laboratory of Metabolic Bone Diseases, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, Hunan, 410011, China.
Clin Oral Investig. 2022 Dec;26(12):7157-7165. doi: 10.1007/s00784-022-04677-6. Epub 2022 Sep 2.
To evaluate the long-term therapeutic effect of EASYDO ACTIVATOR, passive ultrasonic irrigation, and needle irrigation in experimental apical periodontitis in rats.
Sprague-Dawley male rats were used to produce periapical lesions. The pulp chambers of the bilaterally first mandibular molars were exposed and left open for 21 days. The rats were divided into four groups according to different irrigation protocols. Seven days after irrigation, the mandibles were removed for micro-CT, histological, and immunohistochemical analysis. Serum levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were assessed by enzyme-linked immunosorbent assays (ELISA). Statistical data were analyzed by one-way analysis of variance (ANOVA) with LSD tests.
The passive ultrasonic irrigation and EASYDO ACTIVATOR groups had the smallest apical lesions compared to the other groups (P < 0.05), while the needle irrigation group had smaller lesions than the control group (P < 0.05). The EASYDO ACTIVATOR group had less inflammation infiltration compared to the control and needle irrigation groups (P < 0.05). The control and needle irrigation groups had more TNF-α expression compared to the passive ultrasonic irrigation and EASYDO ACTIVATOR groups (P < 0.05). The lowest IL-6 expression was observed in the EASYDO ACTIVATOR group. The EASYDO ACTIVATOR group had the lowest serum level of TNF-α than other groups (P < 0.05). IL-6 expression was significantly lower in the EASYDO ACTIVATOR group in comparison with the control and needle irrigation groups (P < 0.05).
EASYDO ACTIVATOR can significantly reduce the apical lesions and decrease the inflammatory response around the periapical area.
EASYDO ACTIVATOR is recommended for clinical application.
评估 EASYDO ACTIVATOR、被动超声冲洗和针冲洗在大鼠实验性根尖周炎中的长期疗效。
使用 Sprague-Dawley 雄性大鼠制作根尖病变。双侧第一下颌磨牙的牙髓腔被暴露并开放 21 天。根据不同的冲洗方案，将大鼠分为四组。冲洗后 7 天，取下颌进行 micro-CT、组织学和免疫组织化学分析。通过酶联免疫吸附试验（ELISA）测定肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的血清水平。统计数据采用单因素方差分析（ANOVA）和 LSD 检验进行分析。
与其他组相比，被动超声冲洗和 EASYDO ACTIVATOR 组根尖病变最小（P<0.05），而针冲洗组比对照组病变小（P<0.05）。EASYDO ACTIVATOR 组炎症浸润较对照组和针冲洗组少（P<0.05）。与被动超声冲洗和 EASYDO ACTIVATOR 组相比，对照组和针冲洗组 TNF-α表达较多（P<0.05）。EASYDO ACTIVATOR 组 IL-6 表达最低。EASYDO ACTIVATOR 组 TNF-α 血清水平低于其他组（P<0.05）。与对照组和针冲洗组相比，EASYDO ACTIVATOR 组 IL-6 表达明显降低（P<0.05）。
EASYDO ACTIVATOR 可显著减少根尖病变，减轻根尖区周围的炎症反应。
推荐 EASYDO ACTIVATOR 用于临床应用。
