Department of Medical Microbiology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
Department of Chemistry, Faculty of Science, Karadeniz Technical University, Trabzon, Turkey.
Arch Microbiol. 2022 Sep 2;204(9):589. doi: 10.1007/s00203-022-03172-6.
Antibiotic resistance, which has increased rapidly in recent years because of uncontrolled and unconscious antibiotic consumption, poses a major threat to public health. The inadequacy of existing antibiotics has increased the need for new, effective, and less toxic antibiotic raw materials or antibiotic derivatives. Pecan (Carya illinoinensis) and Chestnut (Castanea sativa) flowers possess abundant pollen contents and exhibit similar morphological features. The purpose of this study was to compare these two flower extracts in terms of their antimicrobial and antioxidant activities. Total phenolic content, total flavonoid contents, and phenolic components were also analyzed in aquatic and ethanolic extracts. Antioxidant activities were measured using ferric reducing/antioxidant capacity (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods. Antimicrobial and antifungal activities were compared by means of agar diffusion tests against bacteria including Staphylococcus aureus, Bacillus cereus, Mycobacterium smegmatis, Acinetobacter haemolyticus, and Chromobacterium violaceum, and the yeasts Candida albicans and Candida parapsilosis. Anti-quorum sensing (anti-QS), anti-biofilm, and anti-swarming (SW) activities were also studied against Chromobacterium violaceum ATCC 31532, Chromobacterium violaceum ATCC 12472, and Pseudomonas aeruginosa PA01, respectively. Both extracts were rich in ellagic acid and gallic acid and exhibited similar antioxidant properties. Both flower extracts exhibited high antimicrobial and antifungal activities as well as anti-biofilm, anti-QS, and anti-SW activities.
由于抗生素的无节制和无意识使用，近年来抗生素耐药性迅速增加，对公众健康构成了重大威胁。现有的抗生素已经不足，这增加了对新的、有效和毒性更小的抗生素原料或抗生素衍生物的需求。山核桃（Carya illinoinensis）和板栗（Castanea sativa）花含有丰富的花粉，且具有相似的形态特征。本研究旨在比较这两种花提取物的抗菌和抗氧化活性。还分析了水提物和醇提物中的总酚含量、总黄酮含量和酚类成分。采用铁还原/抗氧化能力（FRAP）和 2,2-二苯基-1-苦基肼基（DPPH）法测定抗氧化活性。通过琼脂扩散试验比较了对包括金黄色葡萄球菌、蜡样芽孢杆菌、耻垢分枝杆菌、溶血不动杆菌和紫色色杆菌在内的细菌，以及白色念珠菌和近平滑念珠菌在内的真菌的抗菌和抗真菌活性。还研究了它们对铜绿假单胞菌 ATCC 31532、ATCC 12472 和铜绿假单胞菌 PA01 的抗群体感应（anti-QS）、抗生物膜和抗 swarm （SW）活性。两种提取物均富含鞣花酸和没食子酸，具有相似的抗氧化特性。两种花提取物均具有较高的抗菌和抗真菌活性以及抗生物膜、抗群体感应和抗 swarm 活性。
