Fontana Riccardo, Caproni Anna, Sicurella Mariaconcetta, Manfredini Stefano, Baldisserotto Anna, Marconi Peggy
Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, 44121 Ferrara, Italy.
Department of Environmental Sciences and Prevention, University of Ferrara, 441211 Ferrara, Italy.
Plants (Basel). 2023 Mar 30;12(7):1508. doi: 10.3390/plants12071508.
pv. is the causal agent of black rot in crucifers, a plant disease with significant economic impact. Xanthomonadaceae is a large family of Gram-negative bacteria that cause symptoms by blocking water flow in plants by invading the xylem. To accomplish this, the main mechanism the bacteria use to adapt to environmental changes and colonize tissues is biofilm formation. In recent years, growing interest in natural antimicrobial compounds has led to the study of different phytocomplexes derived from plants. In this work, was selected, as its leaves are rich in phenols, essential oils, and vitamins that exert antibacterial activity. pv. biofilm, one of its major virulence factors, was studied. Biofilm formation and removal were analyzed on abiotic and biotic surfaces with and without leaf extracts. The data from the analysis show that leaf extracts and single phenols were able to inhibit biofilm growth on abiotic surfaces, but the activity of the whole phytocomplex was significantly higher compared to that of individual phenols. The effect of extracts on cabbage leaves in vivo was also found to be very important, as scanning electron microscopy showed that treatment with the extracts led to clear unblocking of the xylem, implying many advantages for use in black rot control.
野油菜黄单胞菌是十字花科植物黑腐病的病原体，这种植物病害具有重大经济影响。黄单胞菌科是一大类革兰氏阴性细菌，它们通过侵入木质部阻断植物水分流动来引发症状。为实现这一点，细菌用于适应环境变化和定殖组织的主要机制是生物膜形成。近年来，人们对天然抗菌化合物的兴趣日益浓厚，这引发了对源自植物的不同植物复合物的研究。在这项工作中，[具体植物名称]被选中，因为其叶子富含具有抗菌活性的酚类、精油和维生素。对野油菜黄单胞菌的生物膜（其主要毒力因子之一）进行了研究。在有无[具体植物名称]叶提取物的情况下，分析其在非生物和生物表面上生物膜的形成与去除情况。分析数据表明，[具体植物名称]叶提取物和单一酚类能够抑制非生物表面上的生物膜生长，但整个植物复合物的活性相比单个酚类显著更高。还发现[具体植物名称]提取物对甘蓝叶片的体内作用非常重要，因为扫描电子显微镜显示用提取物处理导致木质部明显畅通，这意味着在控制黑腐病方面有诸多优势。
