State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding, China.
College of Life Sciences, Hebei Agricultural University, Baoding, China.
Pest Manag Sci. 2022 Dec;78(12):5113-5123. doi: 10.1002/ps.7129. Epub 2022 Aug 30.
Western flower thrips are considered the major insect pest of horticultural crops worldwide, causing economic and yield loss to Solanaceae crops. The eggplant (Solanum melongena L.) resistance against thrips remains largely unexplored. This work aims to identify thrips-resistant eggplants and dissect the molecular mechanisms underlying this resistance using the integrated metabolomic and transcriptomic analyses of thrips-resistant and -susceptible cultivars.
We developed a micro-cage thrips bioassay to identify thrips-resistant eggplant cultivars, and highly resistant cultivars were identified from wild eggplant relatives. Metabolomic profiles of thrips-resistant and -susceptible eggplant were compared using the gas chromatography-mass spectrometry (GC-MS)-based approach, resulting in the identification of a higher amount of quinic acid in thrips-resistant eggplant compared to the thrips-susceptible plant. RNA-sequencing analysis identified differentially expressed genes (DEGs) by comparing genome-wide gene expression changes between thrips-resistant and -susceptible eggplants. Consistent with metabolomic analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of DEGs revealed that the starch and sucrose metabolic pathway in which quinic acid is a metabolic by-product was highly enriched. External application of quinic acid enhances the resistance of susceptible eggplant to thrips.
Our results showed that quinic acid plays a key role in the resistance to thrips. These findings highlight a potential application of quinic acid as a biocontrol agent to manage thrips and expand our knowledge to breed thrips-resistant eggplant. © 2022 Society of Chemical Industry.
西方花蓟马被认为是世界园艺作物的主要虫害，给茄科作物造成经济和产量损失。茄子对蓟马的抗性在很大程度上尚未被探索。本工作旨在通过对抗蓟马和易感品种的整合代谢组学和转录组学分析，鉴定抗蓟马的茄子，并剖析其抗性的分子机制。
我们开发了一种微笼蓟马生物测定法来鉴定抗蓟马的茄子品种，并从野生茄子近缘种中鉴定出高度抗蓟马的品种。采用基于气相色谱-质谱（GC-MS）的方法比较了抗蓟马和易感茄子的代谢组图谱，结果表明抗蓟马茄子中奎尼酸的含量高于易感植物。通过比较抗蓟马和易感茄子之间的全基因组基因表达变化，RNA-seq 分析鉴定了差异表达基因（DEGs）。与代谢组分析一致，KEGG 通路富集分析表明，奎尼酸是代谢副产物的淀粉和蔗糖代谢途径高度富集。外源施加奎尼酸可增强易感茄子对蓟马的抗性。
我们的结果表明奎尼酸在抗蓟马中起关键作用。这些发现突出了奎尼酸作为生物防治剂来防治蓟马的潜在应用，并扩展了我们对培育抗蓟马茄子的认识。 © 2022 化学工业协会。
