Hu Bijin, Zhao Xiaohui, Lu Yiting, Zhu Yujie, He Hao
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Department of Dermatology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
J Biophotonics. 2022 Dec;15(12):e202200217. doi: 10.1002/jbio.202200217. Epub 2022 Sep 9.
Wound healing is a long-term complex process, in which epidermal stem cells (EPSCs) in epidermis of skin have been found to play an essential role in it. We develop a noninvasive method to activate EPSCs in skin in vivo to promote wound healing, based on a microscopic system to enable a sequential frame-by-frame scanning of a femtosecond laser at 800 nm to the predefined skin region for a single time for 16 seconds. The laser is tightly focused on a submicron spot and localized in the epidermis, and scans point by point to activate EPSCs there. The density and stemness of EPSCs are significantly enhanced for at least 60 hours after the single-time transient photoactivation. We demonstrate this method works in a skin wound mouse model. Our results provide an optical method for in vivo EPSC activation and hold good potential in wound healing.
伤口愈合是一个长期的复杂过程，其中已发现皮肤表皮中的表皮干细胞（EPSCs）在这一过程中发挥着至关重要的作用。我们开发了一种非侵入性方法，基于一个微观系统，能够在体内激活皮肤中的EPSCs以促进伤口愈合，该微观系统可使800nm的飞秒激光对预定义的皮肤区域进行逐帧顺序扫描，单次扫描持续16秒。激光紧密聚焦在一个亚微米光斑上并定位在表皮中，逐点扫描以激活那里的EPSCs。单次瞬态光激活后，EPSCs的密度和干性至少在60小时内显著增强。我们证明了这种方法在皮肤伤口小鼠模型中有效。我们的结果提供了一种体内激活EPSCs的光学方法，在伤口愈合方面具有良好的潜力。
