Gam A A, Neva F A, Krotoski W A
Am J Trop Med Hyg. 1987 Jul;37(1):157-61. doi: 10.4269/ajtmh.1987.37.157.
Sera from 68 patients with parasitologically proven strongyloidiasis were tested by the ELISA and IHA tests using larval antigens prepared from Strongyloides stercoralis and Strongyloides ratti. The ELISA using the S. stercoralis antigen detected the greatest number of sero-reactors (83.8%), whereas the IHA using the S. ratti antigen detected the fewest (55.9%). In addition, the S. stercoralis antigen had higher geometric mean titers than the S. ratti antigen in both the ELISA and the IHA tests. Sera from 37 presumed normal individuals also were tested by IHA and ELISA and nonspecific reactions were seen only with the IHA test. When sera from patients with parasitic infections other than strongyloidiasis were tested, the only consistent cross-reactions were with those sera from patients who had occult filariasis and acute schistosomiasis.
使用从粪类圆线虫和鼠类圆线虫制备的幼虫抗原，通过ELISA和IHA试验对68例经寄生虫学证实为类圆线虫病患者的血清进行检测。使用粪类圆线虫抗原的ELISA检测到的血清反应阳性者数量最多（83.8%），而使用鼠类圆线虫抗原的IHA检测到的数量最少（55.9%）。此外，在ELISA和IHA试验中，粪类圆线虫抗原的几何平均滴度均高于鼠类圆线虫抗原。还通过IHA和ELISA对37名假定正常个体的血清进行检测，仅在IHA试验中出现非特异性反应。对除类圆线虫病以外的寄生虫感染患者的血清进行检测时，唯一一致的交叉反应发生在那些患有隐匿性丝虫病和急性血吸虫病患者的血清中。
