Lindo J F, Conway D J, Atkins N S, Bianco A E, Robinson R D, Bundy D A
Department of Zoology, University of the West Indies, Kingston, Jamaica.
Am J Trop Med Hyg. 1994 Aug;51(2):175-9. doi: 10.4269/ajtmh.1994.51.175.
Recently described enzyme-linked immunosorbent assay (ELISA) and immunoblot methods for the detection of serum IgG against Strongyloides stercoralis larval antigens were prospectively evaluated for the diagnosis of endemic strongyloidiasis. A modification of the ELISA involved preincubation of sera with Onchocerca gutturosa phosphate-buffered saline-soluble extract to remove cross-reactivity with other helminths. The sensitivity of the ELISA increased from 80% to 85% following preincubation. Similarly, there was an increase in specificity from 94% to 97%. The IgG recognition of 41-, 31-, and 28-kD filariform larval components showed sensitivities of 100%, 85%, and 65%, respectively. Both the ELISA following incubation of sera with O. gutturosa extract and serum IgG reactivity to a 41-kD larval component using immunoblotting are sensitive and specific techniques for diagnosing endemic strongyloidiasis.
最近描述的用于检测抗粪类圆线虫幼虫抗原血清IgG的酶联免疫吸附测定（ELISA）和免疫印迹方法，被前瞻性地评估用于地方性粪类圆线虫病的诊断。ELISA的一种改良方法是将血清与盘尾丝虫磷酸盐缓冲盐水可溶性提取物进行预孵育，以消除与其他蠕虫的交叉反应。预孵育后，ELISA的敏感性从80%提高到85%。同样，特异性也从94%提高到97%。对41-kD、31-kD和28-kD丝状幼虫成分的IgG识别敏感性分别为100%、85%和65%。血清与盘尾丝虫提取物孵育后的ELISA以及使用免疫印迹法检测血清IgG对41-kD幼虫成分的反应性，都是诊断地方性粪类圆线虫病的敏感且特异的技术。
