Chemistry Department, College of Science, Jouf University, Sakaka P.O. Box 2014, Saudi Arabia; Chemistry Department, Faculty of Science, Fayoum University, 63514 Fayoum, Egypt.
Biology Department, College of Science, Jouf University, Sakaka P.O. Box 2014, Saudi Arabia; Botany Department, Faculty of Science, Aswan University, Aswan, Egypt.
Ultrason Sonochem. 2022 Sep;89:106152. doi: 10.1016/j.ultsonch.2022.106152. Epub 2022 Aug 29.
Zinc oxide NPs were synthesized solvothermally within sonochemical mediation and characterized by XRD, FTIR, SEM, EDX, elemental mapping, TEM and UV-vis. spectrophotometry. To evaluate the hydroxyl radicals (OH) scavenging activity of arils extract of Egyptian (EGY-PAM) and Yemeni Punica granatum (YEM-PAM), the developed zinc oxide nano particles (ZnO NPs) as a highly productive source of hydroxyl radicals (under Solar-illumination) was used. The yield of OH was trapped and probed via fluorimetric monitoring. This suits the first sensitive/selective photoluminescent avenue to evaluate the OH scavenging activity. The high percentage of DPPH radical scavenging reflected higher contents of phenolics, flavonoids, and anthocyanins that were found in EGY-PAM and YEM-PAM. Although, some secondary metabolites contents were significantly different in EGY-PAM and YEM-PAM, the traditional DPPH radical scavenging methodology revealed insignificant IC. Unlike, the developed fluorimetric probing, sensitively discriminated the OH scavenging activity with IC (105.7 µg/mL) and lower rate of OH productivity (k = 0.031 min) in case of EGY-PAM in comparison to IC (153.4 µg/mL) and higher rate of OH productivity (k = 0.053 min) for YEM-PAM. Our findings are interestingly superior to the TBHQ that is synthetic antioxidant. Moreover, our developed methodology for fluorimetric probing of OH radicals scavenging, recommends EGY-PAM as OH radicals scavenger for diabetic patients while YEM-PAM exhibited a better OH radicals scavenging appropriate for high blood pressure patients. More interestingly, EGY-PAM and YEM-PAM exhibited high anticancer potentiality. The aforementioned OH and DPPH scavenging activities as well as the anticancer potentiality present EGY-PAM and YEM-PAM as promising sources of natural antioxidants, that may have crucial roles in some chronic diseases such as diabetics and hypertension in addition to cancer therapeutic protocols.
氧化锌纳米粒子（ZnO NPs）通过溶剂热法在超声化学介导下合成，并通过 XRD、FTIR、SEM、EDX、元素映射、TEM 和紫外可见分光光度法进行了表征。为了评估埃及（EGY-PAM）和也门石榴（YEM-PAM）种皮提取物清除羟基自由基（OH）的活性，采用开发的氧化锌纳米粒子（ZnO NPs）作为产生羟基自由基（在阳光照射下）的高效源。通过荧光监测捕获和探测 OH 的产量。这适合评估 OH 清除活性的第一个灵敏/选择性光致发光途径。DPPH 自由基清除的高百分比反映了在 EGY-PAM 和 YEM-PAM 中发现的酚类、类黄酮和花青素的含量较高。尽管 EGY-PAM 和 YEM-PAM 中的一些次生代谢物含量存在显著差异，但传统的 DPPH 自由基清除方法揭示了 IC 无显著差异。相比之下，开发的荧光探测灵敏地区分了 OH 清除活性，EGY-PAM 的 IC（105.7μg/mL）和较低的 OH 生成率（k=0.031 min），而 YEM-PAM 的 IC（153.4μg/mL）和较高的 OH 生成率（k=0.053 min）。我们的发现有趣地优于合成抗氧化剂 TBHQ。此外，我们开发的 OH 自由基荧光探测方法推荐 EGY-PAM 作为糖尿病患者的 OH 自由基清除剂，而 YEM-PAM 则表现出更适合高血压患者的 OH 自由基清除活性。更有趣的是，EGY-PAM 和 YEM-PAM 表现出高的抗癌潜力。上述 OH 和 DPPH 清除活性以及抗癌潜力使 EGY-PAM 和 YEM-PAM 成为有前途的天然抗氧化剂来源，它们可能在一些慢性疾病（如糖尿病和高血压）以及癌症治疗方案中发挥关键作用。
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