Department of Basic and Applied Chemistry, Faculty of Science and Technology, University of Central Punjab, Avenue 1, Khayaban-e-Jinnah Road, Johar Town, Lahore 54590, Pakistan.
Department of Medical Education, Sharif Medical and Dental College, Lahore 54000, Pakistan.
Molecules. 2023 Apr 17;28(8):3532. doi: 10.3390/molecules28083532.
Zinc oxide nanoparticles (ZnO NPs) are the second most prevalent metal oxide, owing to their characteristics of low cost, safe, and easily prepared. ZnO NPs have been found to exhibit unique properties which show their potential to be used in various therapies. Numerous techniques have been devised for the manufacture of zinc oxide because it is one of the nanomaterials that has received major research interest. Mushroom sources are proven to be efficient, ecologically friendly, inexpensive, and safe for humankind. In the current study, an aqueous fraction of methanolic extract of () was used to synthesize ZnO NPs. The biosynthesis of ZnO NPs was achieved by using the reducing and capping capability of an aqueous fraction. Bioactive compounds from mushroom, such as flavonoids and polyphenolic compounds, are used in the green synthesis process to biologically reduce metal ions or metal oxides to metal NPs. Biogenically synthesized ZnO NPs were further characterized by using UV-Vis, FTIR, HPLC, XRD, SEM, EDX, zeta sizer and zeta potential analyses. The FTIR showed the functional group at the spectra in the range 3550-3200 cm indicated the presence of the hydroxyl (OH) group, while bands in the range 1720-1706 cm indicated C=O carboxylic stretches bonds. Furthermore, the XRD pattern of ZnO NPs created in the current study was found to be nanocrystals which are hexagonal. The SEM analysis of ZnO NPs showed spherical shapes and size distributions in the range 90-148 nm. Biologically synthesized ZnO NPs have substantial biological activities including antioxidant, antimicrobial, antipyretic, antidiabetic and anti-inflammatory potential. Biological activities showed significant antioxidant (65.7 ± 1.09), antidiabetic (85.18 ± 0.48), and anti-inflammatory potential (86.45 ± 0.60) at 300 µg inhibition in paw inflammation of (1.1 ± 0.06) and yeast-induced pyrexia (97.4 ± 0.51) at 10 mg in a dose-dependent manner. The outcomes of this research indicated that ZnO NPs significantly reduced inflammation and have the ability to scavenge free radicals and prevent protein denaturation, while also indicating their possible use in food and nutraceutical applications to treat various ailments.
氧化锌纳米粒子（ZnO NPs）是第二大常见的金属氧化物，因为它们具有成本低、安全、易于制备等特点。研究发现，ZnO NPs 具有独特的性质，显示出它们在各种治疗中的应用潜力。由于 ZnO 是受到广泛关注的纳米材料之一，因此已经设计了许多制造氧化锌的技术。蘑菇来源被证明是高效、环保、廉价和对人类安全的。在目前的研究中，使用（）甲醇提取物的水相部分来合成 ZnO NPs。通过使用水相部分的还原和封端能力来实现 ZnO NPs 的生物合成。蘑菇中的生物活性化合物，如类黄酮和多酚化合物，用于绿色合成过程中生物还原金属离子或金属氧化物为金属 NPs。生物合成的 ZnO NPs 进一步通过使用 UV-Vis、FTIR、HPLC、XRD、SEM、EDX、zeta 粒度仪和 zeta 电位分析进行表征。FTIR 显示光谱中 3550-3200 cm 范围内的功能团表明存在羟基（OH）基团，而 1720-1706 cm 范围内的带表明 C=O 羧酸伸展键。此外，在当前研究中创建的 ZnO NPs 的 XRD 图案被发现为纳米晶体，是六方晶系的。ZnO NPs 的 SEM 分析显示球形形状和尺寸分布在 90-148nm 范围内。生物合成的 ZnO NPs 具有显著的生物活性，包括抗氧化、抗菌、解热、抗糖尿病和抗炎潜力。生物活性显示出显著的抗氧化（65.7 ± 1.09）、抗糖尿病（85.18 ± 0.48）和抗炎潜力（86.45 ± 0.60），在抑制爪炎症中抑制率为 300µg（1.1 ± 0.06）和酵母诱导发热（97.4 ± 0.51）在剂量依赖性方式下为 10mg。本研究的结果表明，ZnO NPs 显著减轻炎症，并具有清除自由基和防止蛋白质变性的能力，同时也表明它们可能在食品和营养保健品应用中用于治疗各种疾病。
