Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China.
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China.
Food Chem. 2023 Jan 30;400:133904. doi: 10.1016/j.foodchem.2022.133904. Epub 2022 Aug 10.
Resveratrol is a dietary polyphenol that interacts with gut microbiota to possess various biological activities. To identify the microbial metabolites of resveratrol, fresh feces from 12 volunteers were cultured in vitro. Their urine samples were collected after taking a commercial capsule containing 600 mg of resveratrol. Metabolites were characterized and quantified by UPLC-Q-Exactive plus orbitrap MS/MS. The results showed that dihydroresveratrol, 3-(4-hydroxyphenyl)-propionic acid, and lunularin were the major microbial metabolites of RSV with interindividual differences. 3-(4-Hydroxyphenyl)-propionic acid significantly attenuated the inflammatory response of LPS-treated RAW264.7 cells and DSS-induced colitis in antibiotics-treated pseudo-germ-free mice by regulating MAPK and NF-κB pathways. In contrast, dihydroresveratrol did not exhibit significant anti-inflammatory effects, and lunularin exhibited pro-inflammatory effects in cells. This study may help to better understand the health effects of resveratrol and its microbial metabolites.
白藜芦醇是一种膳食多酚，它与肠道微生物群相互作用，具有多种生物活性。为了鉴定白藜芦醇的微生物代谢物，从 12 名志愿者中采集新鲜粪便进行体外培养。在服用含有 600 毫克白藜芦醇的商业胶囊后，收集他们的尿液样本。通过 UPLC-Q-Exactive 加轨道阱 MS/MS 对代谢物进行了表征和定量。结果表明，二氢白藜芦醇、3-(4- 羟基苯基）-丙酸和旋覆花内酯是 RSV 的主要微生物代谢物，具有个体间差异。3-(4- 羟基苯基）-丙酸通过调节 MAPK 和 NF-κB 通路，显著减轻 LPS 处理的 RAW264.7 细胞的炎症反应和抗生素处理的假无菌小鼠的 DSS 诱导的结肠炎。相比之下，二氢白藜芦醇没有表现出显著的抗炎作用，而旋覆花内酯在细胞中表现出促炎作用。本研究可能有助于更好地理解白藜芦醇及其微生物代谢物的健康影响。
