Cepheid, Sunnyvale, California.
Laboratorio Clínico Roe, Lima, Peru.
J Glob Antimicrob Resist. 2022 Dec;31:135-140. doi: 10.1016/j.jgar.2022.08.018. Epub 2022 Aug 31.
Pseudomonas aeruginosa is one of the leading causes of healthcare-associated infections globally. High-risk carbapenemase-encoding P. aeruginosa clones are disseminating in many regions. The aim of this study was to learn more about the lineages and mechanisms of resistance of P. aeruginosa circulating in Peru.
A total of 141 carbapenemase-producing isolates recovered from hospitalized and ambulatory patients in Lima were sequenced and analyzed to infer their lineages through whole-genome sequence typing (wgST) and to identify their antimicrobial resistance genes.
wgST identified nine sequence types (STs); ST111 and ST357 were the most frequently encountered (44.0% and 38.3%, respectively), followed by ST179 (8.5%), with the remaining six detected only sporadically. Among ST357 isolates, 96.3% carried the novel bla allele, whereas the remainder harbored bla. 74.2% of ST111 isolates co-harbored bla and bla, while the rest carried either of these genes individually All other ST lineages carried a single carbapenemase, which was either bla, bla, or bla.
Our study shows that the high-risk P. aeruginosa clones ST357, which harbors the novel bla and ST111, which carries bla and bla have apparently become endemic in the region.
铜绿假单胞菌是全球导致医疗保健相关感染的主要病原体之一。携带高风险碳青霉烯酶基因的铜绿假单胞菌克隆在许多地区传播。本研究旨在更多地了解秘鲁流行的铜绿假单胞菌的谱系和耐药机制。
对利马住院和门诊患者中分离的 141 株产碳青霉烯酶的分离株进行测序和分析，通过全基因组序列分型（wgST）推断其谱系，并鉴定其抗菌药物耐药基因。
wgST 确定了 9 种序列类型（ST）；ST111 和 ST357 最为常见（分别为 44.0%和 38.3%），其次是 ST179（8.5%），其余 6 种仅零星检出。在 ST357 分离株中，96.3%携带新型 bla 等位基因，而其余携带 bla 。74.2%的 ST111 分离株同时携带 bla 和 bla ，而其余的则单独携带其中一种基因。其他所有 ST 谱系均携带一种碳青霉烯酶，分别为 bla 、 bla 或 bla 。
本研究表明，高风险的铜绿假单胞菌克隆 ST357 携带新型 bla ，而 ST111 携带 bla 和 bla ，显然已在该地区流行。
