Key Laboratory of Special Animal Epidemic Disease of Ministry of Agriculture and Rural Affairs, Institute of Special Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, China.
The State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Transbound Emerg Dis. 2022 Nov;69(6):3979-3984. doi: 10.1111/tbed.14693. Epub 2022 Oct 6.
A novel neurological disorder, shaking mink syndrome (SMS), emerged in Denmark and Sweden in 2000. SMS has seldom been reported in China, but the causative agent has not been detected in the country. SMS outbreaks occurred in multiple provinces in 2020. A total of 44 brain samples from minks associated with SMS were collected from Heilongjiang, Liaoning and Shandong provinces of which 28 samples (63.3%) were SMS-astrovirus (SMS-AstV)-positive by reverse transcription PCR. Histopathological examination revealed non-suppurative encephalitis in three minks. Moreover, the complete coding region sequences (CDSs, 6559 bp) of a sample collected from a 2-month-old mink (termed SMS-AstV-H1, GSA accession No. SAMC816786) were amplified by PCR and Sanger sequencing. The complete CDS and open reading frame 2 sequences of SMS-AstV-H1 were 94.3% and 96.4% identical to an SMS-AstV strain (GenBank accession number: GU985458). Phylogenetically, SMS-AstV-H1 was closely related to an SMS-AstV strain (GU985458). Based on the above results, we describe SMS-AstV-associated encephalitis in farmed minks in China. Future studies need to focus on epidemiology, virus isolation and potential interspecies transmission of SMS-AstV.
一种新型的神经紊乱疾病，震颤水貂综合征（SMS），于 2000 年在丹麦和瑞典出现。SMS 在中国很少有报道，但在该国尚未检测到病原体。2020 年，SMS 疫情在多个省份爆发。共从黑龙江、辽宁和山东省的与 SMS 相关的水貂中采集了 44 个脑样本，其中 28 个样本（63.3%）通过反转录 PCR 呈 SMS-astrovirus（SMS-AstV）阳性。组织病理学检查显示 3 只水貂患有非化脓性脑炎。此外，通过 PCR 和 Sanger 测序扩增了从一只 2 个月大的水貂中采集的样本（称为 SMS-AstV-H1，GSA 登录号：SAMC816786）的完整编码区序列（CDS，6559 bp）。SMS-AstV-H1 的完整 CDS 和开放阅读框 2 序列与 SMS-AstV 株（GenBank 登录号：GU985458）的同源性分别为 94.3%和 96.4%。系统发育分析表明，SMS-AstV-H1 与 SMS-AstV 株（GU985458）密切相关。基于上述结果，我们描述了中国养殖水貂中由 SMS-AstV 引起的脑炎。未来的研究需要重点关注 SMS-AstV 的流行病学、病毒分离以及潜在的种间传播。
