Jakubczak Andrzej, Kowalczyk Marek, Mazurkiewicz Ilona, Kondracki Marcin
Institute of Biological Basis of Animal Production, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Lublin, Poland.
Institute of Quality Assessment and Processing of Animal Products, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Lublin, Poland.
Virus Genes. 2021 Jun;57(3):258-265. doi: 10.1007/s11262-021-01834-z. Epub 2021 Apr 15.
Mink astrovirus infection remains a poorly understood disease entity, and the aetiological agent itself causes disease with a heterogeneous course, including gastrointestinal and neurological symptoms. This paper presents cases of astrovirus infection in mink from continental Europe. RNA was isolated from the brains and intestines of animals showing symptoms typical of shaking mink syndrome (n = 6). RT-PCR was used to amplify astrovirus genetic material, and the reaction products were separated on a 1% agarose gel. The specificity of the reaction was confirmed by sequencing fragment coding RdRP protein (length of sequencing product 170 bp) from all samples. The presence of astrovirus RNA was detected in each of the samples tested. Sequencing and bioinformatic analysis indicated the presence of the same variant of the virus in all samples. Comparison of the variant with the sequences available in bioinformatics databases confirmed that the Polish isolates form a separate clade, closely related to Danish isolates. The dissimilarity of the Polish variant to those isolated in other countries ranged from 2.4% (in relation to Danish isolates) to 7.1% (in relation to Canadian isolates). Phylogenetic relationships between variants appear to be associated with the geographic distances between them. To our knowledge, this work describes the first results on the molecular epidemiology of MAstV in continental Europe. The detection of MAstV in Central Europe indicates the need for further research to broaden our understanding of the molecular epidemiology of MAstV in Europe.
水貂星状病毒感染仍然是一种了解甚少的疾病实体，其病原体本身会引发病程各异的疾病，包括胃肠道和神经症状。本文介绍了来自欧洲大陆水貂的星状病毒感染病例。从表现出典型颤抖水貂综合征症状的动物（n = 6）的大脑和肠道中分离出RNA。使用RT-PCR扩增星状病毒遗传物质，并将反应产物在1%琼脂糖凝胶上分离。通过对所有样本中编码RdRP蛋白的片段（测序产物长度170 bp）进行测序，证实了反应的特异性。在所测试的每个样本中均检测到星状病毒RNA的存在。测序和生物信息学分析表明，所有样本中均存在相同的病毒变体。将该变体与生物信息学数据库中可用的序列进行比较，证实波兰分离株形成一个单独的进化枝，与丹麦分离株密切相关。波兰变体与其他国家分离株的差异范围为2.4%（相对于丹麦分离株）至7.1%（相对于加拿大分离株）。变体之间的系统发育关系似乎与它们之间的地理距离有关。据我们所知，这项工作描述了欧洲大陆MAstV分子流行病学的首个结果。在中欧检测到MAstV表明需要进一步研究，以拓宽我们对欧洲MAstV分子流行病学的理解。
