通过 CRISPR/Cas9 同时编辑多个谷蛋白基因高效培育低谷蛋白含量水稻种质资源。

Efficient breeding of low glutelin content rice germplasm by simultaneous editing multiple glutelin genes via CRISPR/Cas9.

机构信息

Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, Jiangsu, China; Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China.

College of Agronomy, Henan Agricultural University, Zhengzhou, Henan 450002, China.

出版信息

Plant Sci. 2022 Nov;324:111449. doi: 10.1016/j.plantsci.2022.111449. Epub 2022 Sep 1.

DOI:10.1016/j.plantsci.2022.111449

PMID:36058302

Abstract

Chronic kidney disease (CKD) and phenylketonuria (PKU) patients need to eat rice with low glutelin content. Therefore, breeding low glutelin content rice varieties with high yield and delicious taste is one of the major goals of rice breeders due to the high demand for the product. In this study, we designed three sgRNAs targeting nine glutelin genes and generated nine T-DNA-free homozygous editing lines with reduced glutelin content compared with the wild-type due to simultaneous mutation(s) in 5-7 glutelin genes. The glutelin content of two lines is even significantly lower than that of the low glutelin content cultivar, LGC-1. Compared to the wild-type, these low glutelin lines showed similar agronomic traits, including yield components and viscosity properties, and can be used as new varieties or parental materials for further breeding.

摘要

慢性肾脏病（CKD）和苯丙酮尿症（PKU）患者需要食用低谷蛋白含量的大米。因此，由于对该产品的高需求，培育产量高、口感好的低谷蛋白含量水稻品种是水稻育种者的主要目标之一。在这项研究中，我们设计了三个针对九个谷蛋白基因的 sgRNA，并生成了九个 T-DNA 自由的纯合编辑系，与野生型相比，由于 5-7 个谷蛋白基因的同时突变，它们的谷蛋白含量降低。两条系的谷蛋白含量甚至明显低于低谷蛋白含量的品种 LGC-1。与野生型相比，这些低谷蛋白系表现出相似的农艺性状，包括产量构成和粘性特性，可作为新品种或亲本材料用于进一步的育种。