Shanghai Agrobiological Gene Center, Shanghai 201106, China.
Genes (Basel). 2024 Nov 20;15(11):1492. doi: 10.3390/genes15111492.
Improving the efficiency of germplasm innovation has always been the aim of rice breeders. Traditional hybrid breeding methods for variety selection rarely meet the practical needs of rice production. The emergence of genome-editing technologies, such as CRISPR/Cas9, provides a new approach to the genetic improvement of crops such as rice. The number of published scientific papers related to "gene editing" and "CRISPR/Cas9" retrievable on websites both from China and other countries exhibited an increasing trend, year by year, from 2014 to 2023. Research related to gene editing in rice accounts for 33.4% and 12.3% of all the literature on gene editing published in China and other countries, respectively, much higher than that on maize and wheat. This article reviews recent research on CRISPR/Cas9 gene-editing technology in rice, especially germplasm innovation and genetic improvement of commercially promoted varieties with improved traits such as disease, insect, and herbicide resistance, salt tolerance, quality, nutrition, and safety. The aim is to provide a reference for the precise and efficient development of new rice cultivars that meet market demand.
提高种质创新效率一直是水稻育种者的目标。传统的杂交选育方法在品种选择上很少能满足水稻生产的实际需要。基因组编辑技术（如 CRISPR/Cas9）的出现为水稻等作物的遗传改良提供了新途径。从 2014 年到 2023 年，在中国和其他国家的网站上可检索到的与“基因编辑”和“CRISPR/Cas9”相关的已发表科学论文数量呈逐年上升趋势。在中国和其他国家发表的所有基因编辑文献中，与基因编辑相关的水稻研究分别占 33.4%和 12.3%，远高于玉米和小麦。本文综述了近年来 CRISPR/Cas9 基因编辑技术在水稻中的研究进展，特别是在具有改良抗病、虫、除草剂抗性、耐盐性、品质、营养和安全性等特性的商业化推广品种的种质创新和遗传改良方面的研究进展。旨在为满足市场需求的精确、高效开发新的水稻品种提供参考。
