National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2023 Jan 3;24(1):849. doi: 10.3390/ijms24010849.
Global food security has benefited from the development and promotion of the two-line hybrid rice system. Excellent eating quality determines the market competitiveness of hybrid rice varieties based on achieving the fundamental requirements of high yield and good adaptability. Developing sterile and restorer lines with improved quality for two-line hybrid breeding by editing quality genes with clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 is an efficient and practical alternative to the lengthy and laborious process of conventional breeding to improve rice quality. We edited and using CRISPR/Cas9 technology to produce both homozygous male sterile mutant lines and homozygous restorer mutant lines with Cas9-free. These mutants have a much lower amylose content while having a significantly higher 2-acetyl-1-pyrroline aroma content. Based on this, a fragrant glutinous hybrid rice was developed without too much effect on most agronomic traits. This study demonstrates the use of CRISPR/Cas9 in creating two-line fragrant glutinous hybrid rice by editing the components of the male sterile and the restorative lines.
全球粮食安全得益于两系杂交水稻体系的发展和推广。优良的食味品质决定了杂交水稻品种的市场竞争力，而这些品种基于实现高产和良好适应性的基本要求。通过编辑簇状规律间隔短回文重复（CRISPR）/Cas9 质量基因，发展具有改良品质的不育系和恢复系，用于两系杂交育种，是一种替代传统育种的高效实用方法，可提高水稻品质。我们使用 CRISPR/Cas9 技术编辑 和 ，产生了不含 Cas9 的纯合雄性不育突变体系和纯合恢复突变体系。这些突变体的直链淀粉含量明显降低，而 2-乙酰-1-吡咯啉香气含量显著增加。在此基础上，开发出了一种香气浓郁的糯稻，对大多数农艺性状的影响不大。本研究通过编辑雄性不育系和恢复系的组成，利用 CRISPR/Cas9 技术创制了两系香型糯稻。
