Analysis of the Adhesive-Invasive Potential of Two Morphologically Different Types of Mycoplasma hominis Colonies.

Mycoplasma hominis is an opportunistic human pathogen that causes acute and chronic infections of the urogenital tract. A new form of M. hominis colonies (microcolonies) was isolated, that differed from typical colonies by morphology, size, growth rate, and resistance to unfavorable factors, in particular, to antibiotics. The formation of microcolonies is associated with a switch in energy metabolism towards nucleoside utilization, which leads to a decrease in energy production and a transition to a persistor-like state. Typical and microcolony cultures of M. hominis H-34 were obtained and a comparative analysis of their adhesive-invasive potential, morphology, and size was carried out. It was shown that both typical and microcolonies can effectively attach and penetrate into HeLa cells. Unlike microcolonies, the morphology and size of cells in typical colonies change significantly after HeLa infection. This indicates functional changes in cells of typical colonies during infection.