Gorina Luisa G, Krylova Natalya A, Rakovskaya Irina V, Geppe Natalia A, Gamova Natalia A, Barkhatova Olga I
Gamaleya National Research Center of Epidemiology and Microbiology, 123098 Moscow, Russia.
Department of Childhood Diseases Sechenov, First Moscow State Medical University (Sechenov University), 119435 Moscow, Russia.
Microorganisms. 2023 Jun 28;11(7):1683. doi: 10.3390/microorganisms11071683.
Improving the management of children with asthma associated with mycoplasma infection is important. Aim: To study the duration of the persistence of antigens, and DNA in a free state, in the structures of circulating immune complexes (CICs) and living cells of and in children with asthma. In total, 205 children with asthma from 1 to 14 years were observed. The reaction of aggregate-hemagglutination (AHAA), the direct immunofluorescence reaction (DIF), the reaction of the polymerase chain reaction (PCR), and the culture method were used. In addition, 47 children were re-examined 1.5 months after the treatment of mycoplasma infection with azithromycin. The number of samples positive for antigens and DNA in the free state and in the structures of CICs significantly decreased. Then, 50 blood serum samples containing antigens, and 50 samples containing antigens were analyzed by culture method. was isolated in 21 (65.5%) of 32 samples containing DNA. was isolated from antigen-positive samples in nine cases. The presented data indicate the long-term persistence of antigens, and DNA of mycoplasma cells in the free state, in the structure of CICs, as well as in the form of "microcolonies". A high level of CICs can be used to predict the course of the disease and the response to therapy.
改善支原体感染相关哮喘儿童的管理非常重要。目的：研究抗原和游离状态的DNA在哮喘儿童循环免疫复合物（CICs）结构及活细胞中的持续存在时间。共观察了205例1至14岁的哮喘儿童。采用凝集-血凝反应（AHAA）、直接免疫荧光反应（DIF）、聚合酶链反应（PCR）和培养方法。此外，47例儿童在用阿奇霉素治疗支原体感染1.5个月后进行了复查。游离状态及CICs结构中抗原和DNA阳性样本数量显著减少。然后，采用培养方法对50份含抗原的血清样本和50份含抗原的样本进行分析。在32份含DNA的样本中，21份（65.5%）分离出。9例从抗原阳性样本中分离出。所呈现的数据表明，抗原和支原体细胞的DNA在游离状态、CICs结构以及“微菌落”形式中可长期持续存在。高水平的CICs可用于预测疾病进程和治疗反应。
