Department of Food and Nutrition, College of BioNano Technology, Gachon University, 1342 Seongnamdaero, Sujeong-gu, Seongnam-si, Gyeonggi-do, 13120, Republic of Korea.
Institute for Aging and Clinical Nutrition Research, Gachon University, Seongnam-si, Gyeonggi-do, 13120, Republic of Korea.
Inflammopharmacology. 2022 Dec;30(6):2261-2272. doi: 10.1007/s10787-022-01060-5. Epub 2022 Sep 5.
The present study aimed to investigate the therapeutic effects of Schisandra chinensis (SC) extract on clinical symptoms of osteoarthritis and the modulating effect on the mechanisms associated with the progression of osteoarthritis in a rat model of monosodium iodoacetate (MIA)-induced osteoarthritis. Osteoarthritis-induced rats were randomized into four groups: MIA injection control (MC), MIA injection with celecoxib (PC), MIA injection with SC extract 100 mg/kg (SC100), and MIA injection with SC extract 200 mg/kg (SC200). Another healthy group received a saline injection as a negative control (NC). During the treatment, weight-bearing measurements were performed once a week for 4 weeks. Histopathological and biochemical analyses of the joints, blood, and chondrocyte tissue were performed following the completion of treatment. Compared with MC rats, SC rats demonstrated significantly alleviated pain behavior, bone erosion, and cartilage degradation. SC reduced serum levels of matrix metalloproteinases and pro-inflammatory cytokines. SC treatment also reversed the levels of biomarkers such as Collagen II and ADAMTS4 in the cartilage tissue. Moreover, SC administration inhibited the phosphorylation levels of nuclear factor kappa B (NF-κB) and NF-κB Inhibitor alpha. This study demonstrates that SC ameliorated osteoarthritis at in vivo level. Our results suggest that SC might be a potential therapeutic agent for osteoarthritis.
本研究旨在探讨五味子（SC）提取物对骨关节炎临床症状的治疗作用，并在碘乙酸单钠（MIA）诱导的骨关节炎大鼠模型中研究其对与骨关节炎进展相关机制的调节作用。骨关节炎诱导大鼠随机分为四组：MIA 注射对照组（MC）、MIA 注射塞来昔布组（PC）、MIA 注射 100mg/kg SC 提取物组（SC100）和 MIA 注射 200mg/kg SC 提取物组（SC200）。另一个健康组接受生理盐水注射作为阴性对照（NC）。在治疗过程中，每周进行一次体重承重测量，共进行 4 周。治疗结束后，对关节、血液和软骨细胞组织进行组织病理学和生化分析。与 MC 大鼠相比，SC 大鼠表现出明显减轻的疼痛行为、骨侵蚀和软骨降解。SC 降低了血清基质金属蛋白酶和促炎细胞因子的水平。SC 治疗还逆转了软骨组织中 Collagen II 和 ADAMTS4 等生物标志物的水平。此外，SC 给药抑制了核因子 kappa B（NF-κB）和 NF-κB 抑制剂 alpha 的磷酸化水平。这项研究表明，SC 在体内水平上改善了骨关节炎。我们的结果表明，SC 可能是骨关节炎的一种潜在治疗剂。
