Karimi Abbas, Ghadiri-Moghaddam Farzaneh, Valipour Masoumeh, Yahyavi Yahya
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Mol Biol Res Commun. 2022 Jun;11(2):67-71. doi: 10.22099/mbrc.2022.42754.1706.
The Human endogenous retroviruses (HERVs) are ancient remnants of exogenous retroviral infections. Their abnormal activation is associated with several diseases, such as cancer and autoimmunity. Epigenetic and environmental factors are probably playing essential roles in the expression of these elements. This study aimed to examine the 96-hour effects of ELF-EMF on HERV-H, K, and W expression in human melanoma cells. SK-MEL-37 cells (the human skin malignant melanoma) were continuously exposed to ELF-EMF (50 Hz) at 1.5 and 3 mT intensity for 96 hours. Following mRNA extraction, the expression level of HERV-H, K, and W was assessed by qPCR. According to our results, exposure to ELF-EMF intensities for 96 hours could significantly downregulate HERV-H, K, and W env gene expression (P<0.001). Our obtained data suggest that low intensity and long-term exposure to ELF-EMF may pave using this type of radiation as a novel therapeutic approach by neutralizing the HERVs upregulated expression in melanoma cells.
人类内源性逆转录病毒（HERVs）是外源性逆转录病毒感染的古老遗迹。它们的异常激活与多种疾病相关，如癌症和自身免疫性疾病。表观遗传和环境因素可能在这些元件的表达中起重要作用。本研究旨在检测极低频电磁场（ELF-EMF）对人黑色素瘤细胞中HERV-H、K和W表达的96小时影响。将SK-MEL-37细胞（人皮肤恶性黑色素瘤细胞）在1.5和3 mT强度下连续暴露于50 Hz的ELF-EMF 96小时。提取mRNA后，通过qPCR评估HERV-H、K和W的表达水平。根据我们的结果，暴露于ELF-EMF强度96小时可显著下调HERV-H、K和W env基因的表达（P<0.001）。我们获得的数据表明，低强度和长期暴露于ELF-EMF可能通过中和黑色素瘤细胞中上调的HERVs表达，为将这种类型的辐射用作一种新的治疗方法铺平道路。
