Zhang Beiying, Bi Qianyu, Huang Shengqi, Lv Siyuan, Zong Xin, Wang Mengran, Ji Xuming
School of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, China.
Weifang Nursing Vocational College, Weifang, China.
Front Pharmacol. 2022 Aug 19;13:914597. doi: 10.3389/fphar.2022.914597. eCollection 2022.
Cancer cachexia is a complex syndrome that leads to an ongoing loss of skeletal muscle mass in many malignant tumors. Our previous studies have evaluated the effectiveness of Baoyuan Jiedu decoction (BJD) in alleviating cancer-induced muscle atrophy. However, the mechanisms of BJD regulating muscle atrophy could not be fully understood. Therefore, we further investigated the mechanisms of BJD mitigating muscle atrophy both in an mouse model and the Lewis-conditioned medium-induced C2C12 myotube atrophy model. We confirmed the quality of BJD extracts by HPLC. In an study, body weight loss and muscle atrophy were alleviated with BJD treatment. GO analysis suggested that ATP metabolism and mitochondria were involved. The results of the electron microscope show that BJD treatment may have a healing effect on mitochondrial structure. Moreover, ATP content and mitochondrial numbers were improved with BJD treatment. Furthermore, both and , we demonstrated that the BJD treatment could improve mitochondrial function owing to the increased number of mitochondria, balanced dynamic, and regulation of the electron transport chain according to the protein and mRNA expressions. In addition, oxidative stress caused by mitochondrial dysfunction was ameliorated by BJD treatment in mice. Consequently, our study provides proof for BJD treatment alleviating cancer cachexia-induced muscle atrophy by modulating mitochondrial function in mice.
癌症恶病质是一种复杂的综合征，会导致许多恶性肿瘤患者的骨骼肌质量持续流失。我们之前的研究评估了保元解毒汤（BJD）在减轻癌症引起的肌肉萎缩方面的有效性。然而，BJD调节肌肉萎缩的机制尚未完全明确。因此，我们进一步研究了BJD在小鼠模型和Lewis条件培养基诱导的C2C12肌管萎缩模型中减轻肌肉萎缩的机制。我们通过高效液相色谱法（HPLC）确认了BJD提取物的质量。在一项研究中，BJD治疗减轻了体重减轻和肌肉萎缩。基因本体（GO）分析表明，ATP代谢和线粒体参与其中。电子显微镜结果显示，BJD治疗可能对线粒体结构有修复作用。此外，BJD治疗提高了ATP含量和线粒体数量。此外，在体内和体外，我们都证明，根据蛋白质和mRNA表达情况，BJD治疗可通过增加线粒体数量、平衡动力学以及调节电子传递链来改善线粒体功能。此外，BJD治疗改善了体内小鼠因线粒体功能障碍引起的氧化应激。因此，我们的研究为BJD治疗通过调节体内小鼠的线粒体功能减轻癌症恶病质引起的肌肉萎缩提供了证据。
