L. 种子二氯甲烷提取物中生物活性成分的抗肥胖潜力。

Antiobesity Potential of Bioactive Constituents from Dichloromethane Extract of L. Seeds.

机构信息

Department of Biotechnology, Lovely Professional University, Phagwara, 144411 Punjab, India.

Department of Biotechnology, Govt. Degree College Kathua, Affiliated to University of Jammu, 184104, J&K (UT), India.

出版信息

Biomed Res Int. 2022 Aug 25;2022:9504787. doi: 10.1155/2022/9504787. eCollection 2022.

DOI:10.1155/2022/9504787

PMID:36060144

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9436577/

Abstract

PURPOSE

Effectively controlling the accumulation of adipose tissue can be a therapeutic strategy for treating obesity, which is a global problem. The present study was designed for comparative assessment of antiobesity activities of the -dichloromethane seed extract (DCME) and the isolated phytochemicals, bakuchiol, isopsoralen, and psoralen, through antiadipogenesis and pancreatic lipase (PL) inhibition assays. pancreatic lipase activity was determined spectrophotometrically by measuring the hydrolysis of -nitrophenyl butyrate (-NPB) to -nitrophenol at 405 nm, and adipogenesis was assayed in 3 T3-L1 adipocytes (by using Oil Red O staining) using -dichloromethane seed extract (DCME) and individual compounds, isolated from the extract.

RESULT

Antilipase as well as antiadipogenesis activity was displayed by both the DCME and the compounds. Maximum antilipase property was recorded in DCME (26.02 ± .041%) at 100 g/ml, while, among the isolated compounds, bakuchiol exhibited a higher activity (24.2 ± 0.037%) at 100 g/ml concentration, compared to other isolates. DCME was found to exhibit antiadipogenesis property, 75 ± 0.003% lipid accumulation, compared to the control at 100 g/ml dose. Bakuchiol, isopsoralen, and psoralen inhibited the lipid accumulation in 3T3-L1 preadipocytes, 78.06 ± 0.002%, 80.91 ± 0.004%, and 80.91 ± 0.001%, respectively, lipid accumulation in comparison to control at 25 M dose.

CONCLUSION

The present study highlights the antiobesity potential of and its active constituents. Thus, it can be concluded that has the potential to treat obesity and related diseases; however, further research on dose standardization and clinical trials are required.

摘要

目的

有效控制脂肪组织的积累可能是治疗肥胖的一种治疗策略，肥胖是一个全球性问题。本研究旨在通过抗脂肪生成和胰腺脂肪酶（PL）抑制试验，对 -二氯甲烷种子提取物（DCME）和分离的植物化学物质巴库醇、异补骨脂素和补骨脂素的抗肥胖活性进行比较评估。通过测量 -硝基苯丁酸（-NPB）水解为 -硝基苯酚在 405nm 处的吸光度，用分光光度法测定胰腺脂肪酶活性，并用 -二氯甲烷种子提取物（DCME）和从提取物中分离的单个化合物在 3T3-L1 脂肪细胞中（通过油红 O 染色）测定脂肪生成。

结果

DCME 和化合物均表现出抗脂肪酶和抗脂肪生成活性。在 100μg/ml 时，DCME 表现出最大的抗脂肪酶特性（26.02±0.041%），而在分离的化合物中，巴库醇在 100μg/ml 浓度下表现出更高的活性（24.2±0.037%），与其他分离物相比。与对照组相比，DCME 在 100μg/ml 剂量下表现出抗脂肪生成特性，脂质积累为 75±0.003%。巴库醇、异补骨脂素和补骨脂素分别在 25μM 剂量下抑制 3T3-L1 前脂肪细胞的脂质积累，脂质积累为 78.06±0.002%、80.91±0.004%和 80.91±0.001%。