Guo Jia, Zhu Jiale, Zhao Tianyi, Sun Zhihua, Song Shengnan, Zhang Yu, Zhu Dexin, Cao Shuzhu, Deng Xingmei, Chai Yingjin, Sun Yongxue, Maratbek Suleimenov, Chen Chuangfu, Liu Liangbo, Zhang Hui
State International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi, China.
Collaborative Innovation Center for Sheep Healthy Farming and Zoonotic Disease Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Front Microbiol. 2022 Aug 17;13:968592. doi: 10.3389/fmicb.2022.968592. eCollection 2022.
can inhabit hostile environments, including osmotic stress. How responds collectively to osmotic stress is largely unexplored, particularly in spatially structured communities such as a biofilm. To gain insight into this growth mode, we set out to characterize the 16M biofilm, describe its phenotype, and carry out a comparative transcriptomic analysis between biofilms under osmotic stress and control conditions. We determined that the bacteria challenged with 1.5 M NaCl had a reduced ability to aggregate and form clumps and develop a biofilm; however, the salt stress promoted the release of the outer membrane vesicles from the biofilm. Together with the genotypical response to osmotic stress, we identified 279 differentially expressed genes in 16M grown under osmotic conditions compared with control conditions; 69 genes were upregulated and 210 downregulated. Under osmotic stress, the main changed genes of biofilm were predicted to be involved in flagellar assembly, cell envelope, translation, small RNA regulation, transport and binding proteins, and energy metabolism. In addition, the ABC transporter was enriched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. We highlight 12 essential ABC transporter genes associated with a bacterial response to osmotic stress at the biofilm stage, including one specific locus, BME_RS12880, mediating betaine accumulation in biofilms to eliminate osmotic stress. The current study results can help researchers gain insights into 16M biofilm adaptation to osmotic stress and provide information for developing intervention strategies to control .
能够在恶劣环境中生存，包括渗透胁迫环境。细菌如何集体应对渗透胁迫在很大程度上尚未得到探索，特别是在生物膜等空间结构群落中。为了深入了解这种生长模式，我们着手对16M生物膜进行表征，描述其表型，并对渗透胁迫下的生物膜与对照条件下的生物膜进行比较转录组分析。我们确定，用1.5M NaCl处理的细菌聚集、形成团块并形成生物膜的能力降低；然而，盐胁迫促进了生物膜中外膜囊泡的释放。结合对渗透胁迫的基因型反应，我们在渗透条件下生长的16M中鉴定出279个与对照条件相比差异表达的基因；69个基因上调，210个基因下调。在渗透胁迫下，预测生物膜中主要变化的基因参与鞭毛组装、细胞包膜、翻译、小RNA调控、转运和结合蛋白以及能量代谢。此外，ABC转运蛋白在基因本体论（GO）和京都基因与基因组百科全书（KEGG）途径中富集。我们强调了12个与生物膜阶段细菌对渗透胁迫反应相关的必需ABC转运蛋白基因，包括一个特定位点BME_RS12880，它介导生物膜中甜菜碱的积累以消除渗透胁迫。目前的研究结果可以帮助研究人员深入了解16M生物膜对渗透胁迫的适应性，并为制定控制策略提供信息。
