用于从环境样本中快速初步鉴定霍乱弧菌的多种检测培养基的评估。
Evaluation of the multitest medium for rapid presumptive identification of Vibrio cholerae from environmental sources.
作者信息
Nair G B, Misra S, Bhadra R K, Pal S C
出版信息
Appl Environ Microbiol. 1987 May;53(5):1203-5. doi: 10.1128/aem.53.5.1203-1205.1987.
Abstract
The multitest V. cholerae medium (VC medium) for rapid presumptive identification of Vibrio cholerae was evaluated. On the basis of reactions in the VC medium, 379 strains recovered during a yearlong ecological study in Calcutta were presumptively identified as V. cholerae. Further phenotypic characterization of these strains revealed that the reactions of 371 (97.9%) isolates were consistent with that of V. cholerae. False-positive reactions were exhibited by eight (2.1%) strains, three of which were identified as Vibrio fluvialis biotype 1. By slightly varying the basic formulation of the VC medium, we could eliminate some false-positive reactions. On the basis of the present evaluation, we recommend the routine use of the VC medium.
摘要
对用于快速初步鉴定霍乱弧菌的多测试霍乱弧菌培养基（VC 培养基）进行了评估。根据在 VC 培养基中的反应，在加尔各答为期一年的生态学研究中回收的 379 株菌株被初步鉴定为霍乱弧菌。对这些菌株的进一步表型特征分析表明，371 株（97.9%）分离株的反应与霍乱弧菌一致。8 株（2.1%）菌株出现假阳性反应，其中 3 株被鉴定为河流弧菌生物型 1。通过略微改变 VC 培养基的基本配方，我们可以消除一些假阳性反应。基于目前的评估，我们建议常规使用 VC 培养基。
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