Enhances Nasopharyngeal Carcinoma Cell Radiosensitivity the //Signal Transducer and Activator of Transcription-1/Forkhead Box O-1 Axis.

Nasopharyngeal carcinoma (NPC) is a common malignancy of the nasopharynx, and radioresistant represents the main obstacle in NPC treatment. Malignant transformation of normal cells is driven by genetic and epigenetic changes, which are primarily manifested as changes in miRNA levels and DNA methylation status. microRNA (miR)-613 plays an inhibitory role in several types of cancer. Herein, the current study sought to explore the roles of in NPC cell radiosensitivity. expression patterns in NPC tissues were detected, and its correlation with clinical indexes was analyzed. NP-69 and C666-1 cell lines were selected for cellular experimentation. Radioresistant cell line C666-1R was obtained by fractionated radiation. Cell viability, survival fraction, and apoptosis were detected by CCK-8, colony formation assay, and flow cytometry. The binding relation between and was verified by dual-luciferase and RIP assays. was lowly expressed in NPC tissues and cells, with lower expression levels in C666-1R than C666-1, and further correlated with lymph node metastasis, tumor size, and tumor metastasis. overexpression reduced C666-1R cell viability and survival fraction and increased apoptosis, while C666-1 cells with silencing presented the opposite trends. targeted . and overexpression led to enhanced C666-1R cell viability and survival fraction and decreased apoptosis. reduced methylation and elevated protein level by inhibiting . enhanced NPC radiosensitivity by inhibiting the //STAT1/FOXO1 pathway. Collectively, inhibited , reduced methylation, and increased protein level, thus inhibiting the STAT1/FOXO1 pathway and enhancing the radiosensitivity of NPC cells.