The Northwest Normal University, Lanzhou, China.
Gansu Provincial Maternity and Child-Care Hospital, Lanzhou, China.
PeerJ. 2022 Aug 30;10:e13949. doi: 10.7717/peerj.13949. eCollection 2022.
The difference of metabolites in medicinal plants has always been concerned to be influenced by external environmental factors. However, the relationship between endophytes and host metabolites remains unclear.
In this study, we used 16S and ITS amplicon sequencing to compare endophyte diversity among different tissue types and ages of . Endophyte diversity and abundance was also analyzed in relation to the abundance of four secondary metabolites (Gentiopicroside, Loganic acid, Swertiamarine and Sweroside).
The diversity and richness of endophyte differed as a function of tissue types and ages. Four metabolites of were significantly correlated with the abundance of dominant endophyte genera. The predictive function analysis showed that metabolism was main function of endophytic bacteria in different tissue and year root samples, while saprotroph was dominant trophic modes of endophytic fungi in the different year root samples. The dominant trophic modes of endophytic fungi was saprotroph and pathotroph, and relative abundances differed in the different tissue samples. The results of this study will help to elucidate the plant-microbial interactions and provide key information on the role of endophytes in the production of and its important metabolites.
药用植物代谢产物的差异一直受到外界环境因素的影响。然而，内共生菌与宿主代谢产物之间的关系尚不清楚。
本研究采用 16S 和 ITS 扩增子测序比较不同组织类型和年龄的 内共生菌的多样性。还分析了内共生菌的多样性和丰度与四种次生代谢物（龙胆苦苷、獐牙菜苦苷、当药苦苷和当药苷）丰度的关系。
内共生菌的多样性和丰富度随组织类型和年龄的不同而不同。 中的四种代谢物与优势内共生菌属的丰度显著相关。预测功能分析表明，代谢是不同组织和年生根样本中内生细菌的主要功能，而腐生是不同年生根样本中内生真菌的主要营养模式。内生真菌的主要营养模式是腐生和寄生，在不同的组织样本中相对丰度不同。本研究的结果将有助于阐明植物-微生物相互作用，并为内生菌在 和其重要代谢物产生中的作用提供关键信息。
