Benson Muthee Kanji, Maitho Timothy E, Kanja Laetitia Wakonyu, Onyancha Jared Misonge
Department of Public Health, Pharmacology & Toxicology, Faculty of Veterinary Medicine, University of Nairobi, P.O. Box 29053-00625, Nairobi, Kenya.
Department of Pharmacognosy, School of Pharmacy, Mount Kenya University, P.O Box 342-0100, Thika, Kenya.
Evid Based Complement Alternat Med. 2022 Aug 25;2022:7393537. doi: 10.1155/2022/7393537. eCollection 2022.
Over 80% of cultural societies in low-income countries use plant preparations in traditional medicine with unknown potency and safety profiles. root extracts are used by some Kenyan herbalists. However, the claims of the plant to remove retained placenta during birth have remained uninvestigated. Therefore, the current study evaluated its uterotonic activities. Acute toxicity in Wistar rats and the phytochemical composition of the plant were also studied. The plant was collected from Embu County in Kenya. The water and ethanol extracts were prepared by maceration. Uterine strips were isolated from primed mature female Wistar rats and used to study the uterotonic activities of the extracts. De Jalon's solution and oxytocin were used as negative and positive controls, respectively. Acute oral toxicity studies were done following the OECD 423 guideline and phytochemical screening were based on standard phytochemical procedures. The study met all the approval requirements before commencement. Data obtained from the uterotonic activity were analysed by using GraphPad Prism Version 8.0.1 software and expressed as a percentage increase or decrease of mean as mean ± SEM relative to the controls. The findings of acute oral toxicity were expressed using LD. Additionally, the phytochemical components of the were tabulated. The uterotonic effect of root water extract was higher than that of ethanol extract. A single dose of the root water extract at 2000 mg/kg did not cause mortality in the tested Wistar rats. Besides, there were no changes in hematological and biochemical parameters. The extracts did not reveal changes in the gross morphology of the liver, kidney, heart, and lung of the tested Wistar rats. However, the histopathological studies of root water extracts exhibited toxicity in the liver, kidney, and lung tissues of Wistar rats at a concentration of 2000 mg/kg. Alkaloids, glycosides, saponins, phytosterols, terpenes, proteins, phenols, and oils were recorded in The findings from this study provided scientific evidence which is useful in validating the use of extracts in the stimulation of the uterus during birth.
在低收入国家，超过80%的文化社群在传统医学中使用植物制剂，但其效力和安全性未知。一些肯尼亚草药医生使用[植物名称]根提取物。然而，该植物在分娩时用于排出胎盘滞留的说法尚未得到研究。因此，本研究评估了其宫缩活性。还研究了该植物对Wistar大鼠的急性毒性及其植物化学成分。该植物采自肯尼亚的恩布县。通过浸渍法制备水提取物和乙醇提取物。从经预处理的成年雌性Wistar大鼠中分离出子宫条，用于研究提取物的宫缩活性。分别使用德贾隆溶液和缩宫素作为阴性和阳性对照。按照经合组织423指南进行急性口服毒性研究，植物化学筛选基于标准的植物化学程序。该研究在开始前满足所有审批要求。从宫缩活性获得的数据使用GraphPad Prism 8.0.1版软件进行分析，并表示为相对于对照组平均值的百分比增减，以平均值±标准误表示。急性口服毒性的结果用半数致死量（LD）表示。此外，[植物名称]的植物化学成分被制成表格。[植物名称]根水提取物的宫缩作用高于乙醇提取物。在受试的Wistar大鼠中，单剂量2000mg/kg的[植物名称]根水提取物未导致死亡。此外，血液学和生化参数没有变化。提取物未显示受试Wistar大鼠的肝脏、肾脏、心脏和肺的大体形态有变化。然而，[植物名称]根水提取物的组织病理学研究显示，在2000mg/kg浓度下，Wistar大鼠的肝脏、肾脏和肺组织出现毒性。在[植物名称]中记录到生物碱、糖苷、皂苷、植物甾醇、萜类、蛋白质、酚类和油类。本研究的结果提供了科学证据，有助于验证[植物名称]提取物在分娩时刺激子宫方面的应用。
