是胶质母细胞瘤的预后标志物，与肿瘤浸润免疫细胞和氧化应激有关。
Is a Prognostic Marker for Glioblastoma Associated with Tumor-Infiltrating Immune Cells and Oxidative Stress.
机构信息
Department of Clinical Biobank & Institute of Oncology, Affiliated Hospital of Nantong University, Nantong, China 226000.
Department of Neurosurgery, Affiliated Hospital of Nantong University, China 226000.
出版信息
Oxid Med Cell Longev. 2022 Aug 24;2022:6711085. doi: 10.1155/2022/6711085. eCollection 2022.
BACKGROUND
encodes the small subunit A of serine palmitoyltransferase. It catalyzes the formation of sphingoid long-chain base backbone of sphingolipids. Its role in glioma prognosis and tumor-infiltrating immune cells remains unclear.
METHODS
We analyzed expression and association with clinical prognosis using GEPIA and CGGA database. Then, GSEA was performed to identify relevant biological functions of . The correlations between expression and tumor immune infiltrates were investigated using CIBERSORT and TIMER. Finally, IHC and IF were performed to confirm the value of prognosis and the correlation with immune infiltration.
RESULTS
expression was significantly upregulated in diffuse glioma compared to normal tissues and associated with poor survival in GEPIA and CGGA database. Then, we identified biological processes and signaling pathways associated with expression. The result showed that enriched in the GO term like oxidative stress. Finally, we showed that expression was significantly associated with tumor-infiltrating immune cells and overall survival via IHC.
CONCLUSION
These findings suggest that expression might be used as a prognostic biomarker for glioma and potential target for novel glioma therapy.
背景
编码丝氨酸棕榈酰转移酶小亚基 A。它催化鞘脂类的鞘氨醇长链碱基骨架的形成。其在神经胶质瘤预后和肿瘤浸润免疫细胞中的作用尚不清楚。
方法
我们使用 GEPIA 和 CGGA 数据库分析了表达情况及其与临床预后的关联。然后，进行 GSEA 以确定 的相关生物学功能。使用 CIBERSORT 和 TIMER 研究了 表达与肿瘤免疫浸润的相关性。最后，通过 IHC 和 IF 验证了预后的价值及其与免疫浸润的相关性。
结果
与正常组织相比，弥漫性神经胶质瘤中表达显著上调，并在 GEPIA 和 CGGA 数据库中与不良生存相关。然后，我们确定了与 表达相关的生物学过程和信号通路。结果表明，在 GO 术语中富集了氧化应激等。最后，我们通过 IHC 表明 表达与肿瘤浸润免疫细胞和总生存显著相关。
结论
这些发现表明， 表达可能可作为神经胶质瘤的预后生物标志物和新型神经胶质瘤治疗的潜在靶点。
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