Institute of Applied Biology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovak Republic.
Institute of Food Sciences, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovak Republic.
Res Vet Sci. 2022 Dec 20;152:261-269. doi: 10.1016/j.rvsc.2022.08.022. Epub 2022 Aug 31.
Sea buckthorn (Hippophae rhamnoides L.) is described by various beneficial effects as it contains several bioactive substances characterized by antioxidant effects. These effects are closely related to the reduction of oxidative stress that is involved in the development of the disease. One such diseases is Diabetes mellitus, the prevalence of which is growing and is associated primarily with diet, lack of exercise and/or genetics. This study intends to examine the effects of sea buckthorn and metformin on body weight, water and feed intake, glycaemia, insulinemia, sorbitol accumulation and cataract development in Zucker diabetic fatty rats, which represent an animal model of type 2 Diabetes mellitus, as well as to characterize the individual content of bioactive substances and the antioxidant activity of sea buckthorn. Particular concentrations were applied (500 and 1000 mg.kg body weight of sea buckthorn, and combinations with 150 mg.kg body weight of metformin) by gastric gavage. The total antioxidant capacity and bioactive compounds were determined by spectrophotometric analysis. The best results of the study showed suppression of hyperglycaemia, water intake, decreased sorbitol levels in the lens of the eyes after sea buckthorn treatment. Determination of bioactive compounds showed significantly higher values in dry berries when compared to fresh berries of sea buckthorn and high total antioxidant capacity. Our results represent an interest in sea buckthorn and its potential use in the treatment of Diabetes mellitus as well as other experimental studies.
沙棘（Hippophae rhamnoides L.）因其含有多种具有抗氧化作用的生物活性物质而具有多种有益作用。这些作用与氧化应激的减少密切相关，氧化应激与疾病的发展有关。其中一种疾病是糖尿病，其患病率正在上升，主要与饮食、缺乏运动和/或遗传有关。本研究旨在研究沙棘和二甲双胍对 Zucker 糖尿病肥胖大鼠体重、水和饲料摄入、血糖、胰岛素血症、山梨醇积累和白内障发展的影响，Zucker 糖尿病肥胖大鼠是 2 型糖尿病的动物模型，以及表征沙棘的生物活性物质的个体含量和抗氧化活性。通过胃灌胃应用特定浓度（500 和 1000 mg.kg 体重的沙棘，以及与 150 mg.kg 体重的二甲双胍组合）。总抗氧化能力和生物活性化合物通过分光光度分析确定。研究的最佳结果表明，沙棘处理可抑制高血糖、水摄入、眼睛晶状体中山梨醇水平降低。生物活性化合物的测定表明，与新鲜沙棘浆果相比，干浆果中的值明显更高，总抗氧化能力也更高。我们的研究结果代表了对沙棘及其在治疗糖尿病以及其他实验研究中的潜在用途的兴趣。
