Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology (SUSTech), Shenzhen, Guangdong 518055, China.
Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology (SUSTech), Shenzhen, Guangdong 518055, China.
Curr Opin Plant Biol. 2022 Oct;69:102294. doi: 10.1016/j.pbi.2022.102294. Epub 2022 Sep 2.
Following transcription initiation, RNA polymerase II (Pol II) elongates through the genic region and terminates after the polyadenylation signal. This process is accompanied by splicing, 3' cleavage, and polyadenylation, to eventually form a mature mRNA. Recent advances in short-read and long-read high-throughput sequencing methods have shed light on the global landscape of these co-transcriptional events at nucleotide resolution. In this mini review, we summarize recent developments in genome-wide approaches that broadened our understanding of nascent RNA processing in plants.
转录起始后，RNA 聚合酶 II（Pol II）通过基因区域延伸，在多聚腺苷酸化信号后终止。这一过程伴随着剪接、3' 切割和多聚腺苷酸化，最终形成成熟的 mRNA。短读长和长读长高通量测序方法的最新进展揭示了这些转录共发生事件在核苷酸分辨率下的全局图谱。在这篇迷你综述中，我们总结了近年来在全基因组方法方面的进展，这些进展拓宽了我们对植物新生 RNA 加工的理解。
