• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

TRIM-NHL 蛋白 NHL-2 调节秀丽隐杆线虫生殖系中的细胞命运选择。

TRIM-NHL protein, NHL-2, modulates cell fate choices in the C. elegans germ line.

机构信息

Department of Genetics, Washington University School of Medicine, St. Louis, MO, 63110, USA.

Department of Biology, Syracuse University, Syracuse, NY, 13210, USA.

出版信息

Dev Biol. 2022 Nov;491:43-55. doi: 10.1016/j.ydbio.2022.08.010. Epub 2022 Sep 3.

DOI:10.1016/j.ydbio.2022.08.010
PMID:36063869
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9922029/
Abstract

Many tissues contain multipotent stem cells that are critical for maintaining tissue function. In Caenorhabditis elegans, germline stem cells allow gamete production to continue in adulthood. In the gonad, GLP-1/Notch signaling from the distal tip cell niche to neighboring germ cells activates a complex regulatory network to maintain a stem cell population. GLP-1/Notch signaling positively regulates production of LST-1 and SYGL-1 proteins that, in turn, interact with a set of PUF/FBF proteins to positively regulate the stem cell fate. We previously described sog (suppressor of glp-1 loss of function) and teg (tumorous enhancer of glp-1 gain of function) genes that limit the stem cell fate and/or promote the meiotic fate. Here, we show that sog-10 is allelic to nhl-2. NHL-2 is a member of the conserved TRIM-NHL protein family whose members can bind RNA and ubiquitinate protein substrates. We show that NHL-2 acts, at least in part, by inhibiting the expression of PUF-3 and PUF-11 translational repressor proteins that promote the stem cell fate. Two other negative regulators of stem cell fate, CGH-1 (conserved germline helicase) and ALG-5 (Argonaute protein), may work with NHL-2 to modulate the stem cell population. In addition, NHL-2 activity promotes the male germ cell fate in XX animals.

摘要

许多组织都含有多能干细胞，这些细胞对于维持组织功能至关重要。在秀丽隐杆线虫中，生殖干细胞允许配子在成年期继续产生。在性腺中，来自远端顶端细胞龛的 GLP-1/Notch 信号传递到邻近的生殖细胞，激活一个复杂的调控网络来维持干细胞群体。GLP-1/Notch 信号正向调节 LST-1 和 SYGL-1 蛋白的产生，而这些蛋白又与一组 PUF/FBF 蛋白相互作用，正向调节干细胞命运。我们之前描述了 sog（GLP-1 功能丧失的抑制物）和 teg（GLP-1 功能获得的肿瘤增强物）基因，这些基因限制干细胞命运并/或促进减数分裂命运。在这里，我们表明 sog-10 与 nhl-2 是等位基因。NHL-2 是保守的 TRIM-NHL 蛋白家族的成员，其成员可以结合 RNA 和泛素化蛋白质底物。我们表明 NHL-2 至少部分通过抑制 PUF-3 和 PUF-11 翻译抑制蛋白的表达来发挥作用，这些蛋白促进干细胞命运。另外两个干细胞命运的负调控因子，CGH-1（保守的生殖系解旋酶）和 ALG-5（Argonaute 蛋白），可能与 NHL-2 一起调节干细胞群体。此外，NHL-2 活性促进 XX 动物中的雄性生殖细胞命运。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/077e8a997942/nihms-1866074-f0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/502d52dfa305/nihms-1866074-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/52e844a7857c/nihms-1866074-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/6921f1229ddc/nihms-1866074-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/a8e8ab8421c7/nihms-1866074-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/02effa3f15fb/nihms-1866074-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/3c18dc17ace9/nihms-1866074-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/5a4f1d500d57/nihms-1866074-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/20695f775e33/nihms-1866074-f0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/077e8a997942/nihms-1866074-f0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/502d52dfa305/nihms-1866074-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/52e844a7857c/nihms-1866074-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/6921f1229ddc/nihms-1866074-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/a8e8ab8421c7/nihms-1866074-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/02effa3f15fb/nihms-1866074-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/3c18dc17ace9/nihms-1866074-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/5a4f1d500d57/nihms-1866074-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/20695f775e33/nihms-1866074-f0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d89b/9922029/077e8a997942/nihms-1866074-f0009.jpg

相似文献

1
TRIM-NHL protein, NHL-2, modulates cell fate choices in the C. elegans germ line.TRIM-NHL 蛋白 NHL-2 调节秀丽隐杆线虫生殖系中的细胞命运选择。
Dev Biol. 2022 Nov;491:43-55. doi: 10.1016/j.ydbio.2022.08.010. Epub 2022 Sep 3.
2
GLP-1 Notch-LAG-1 CSL control of the germline stem cell fate is mediated by transcriptional targets lst-1 and sygl-1.GLP-1 Notch-LAG-1 CSL 通过转录靶标 lst-1 和 sygl-1 来控制生殖干细胞命运。
PLoS Genet. 2020 Mar 20;16(3):e1008650. doi: 10.1371/journal.pgen.1008650. eCollection 2020 Mar.
3
PUF-8, a Pumilio homolog, inhibits the proliferative fate in the Caenorhabditis elegans germline.PUF-8，一种 Pumilio 同源物，抑制秀丽隐杆线虫生殖系中的增殖命运。
G3 (Bethesda). 2012 Oct;2(10):1197-205. doi: 10.1534/g3.112.003350. Epub 2012 Oct 1.
4
Subunits of the DNA polymerase alpha-primase complex promote Notch-mediated proliferation with discrete and shared functions in C. elegans germline.DNA 聚合酶 α-引发酶复合物的亚基在秀丽隐杆线虫生殖系中以不同和共同的功能促进 Notch 介导的增殖。
FEBS J. 2018 Jul;285(14):2590-2604. doi: 10.1111/febs.14512. Epub 2018 May 28.
5
A PUF Hub Drives Self-Renewal in Germline Stem Cells.PUF 集线器驱动生殖干细胞自我更新。
Genetics. 2020 Jan;214(1):147-161. doi: 10.1534/genetics.119.302772. Epub 2019 Nov 18.
6
SYGL-1 and LST-1 link niche signaling to PUF RNA repression for stem cell maintenance in Caenorhabditis elegans.SYGL-1和LST-1将龛信号传导与PUF RNA抑制相联系，以维持秀丽隐杆线虫的干细胞。
PLoS Genet. 2017 Dec 12;13(12):e1007121. doi: 10.1371/journal.pgen.1007121. eCollection 2017 Dec.
7
Germline stem cells and their regulation in the nematode Caenorhabditis elegans.线虫秀丽隐杆线虫中的生殖干细胞及其调控。
Adv Exp Med Biol. 2013;786:29-46. doi: 10.1007/978-94-007-6621-1_3.
8
A sensitized genetic screen to identify regulators of Caenorhabditis elegans germline stem cells.一种用于鉴定秀丽隐杆线虫生殖干细胞调控因子的敏感遗传筛选方法。
G3 (Bethesda). 2022 Mar 4;12(3). doi: 10.1093/g3journal/jkab439.
9
Germline Stem Cell Differentiation Entails Regional Control of Cell Fate Regulator GLD-1 in Caenorhabditis elegans.生殖系干细胞分化需要秀丽隐杆线虫中细胞命运调节因子GLD-1的区域控制。
Genetics. 2016 Mar;202(3):1085-103. doi: 10.1534/genetics.115.185678. Epub 2016 Jan 12.
10
Analysis of Germline Stem Cell Differentiation Following Loss of GLP-1 Notch Activity in Caenorhabditis elegans.秀丽隐杆线虫中GLP-1 Notch活性丧失后生殖系干细胞分化的分析
Genetics. 2015 Sep;201(1):167-84. doi: 10.1534/genetics.115.178061. Epub 2015 Jul 8.

引用本文的文献

1
A noncanonical Pol III-dependent, Microprocessor-independent biogenesis pathway generates a germline enriched miRNA family.一条非经典的依赖于Pol III且不依赖于微处理器的生物合成途径产生了一个在生殖系中富集的miRNA家族。
bioRxiv. 2025 Jun 2:2025.04.11.648421. doi: 10.1101/2025.04.11.648421.
2
Germline as Three Distinct Tumor Models.种系作为三种不同的肿瘤模型。
Biology (Basel). 2024 Jun 8;13(6):425. doi: 10.3390/biology13060425.
3
COP9 signalosome component CSN-5 stabilizes PUF proteins FBF-1 and FBF-2 in Caenorhabditis elegans germline stem and progenitor cells.

本文引用的文献

1
Release of CHK-2 from PPM-1.D anchorage schedules meiotic entry.CHK-2从PPM-1.D锚定物的释放安排减数分裂进入。
Sci Adv. 2022 Feb 18;8(7):eabl8861. doi: 10.1126/sciadv.abl8861. Epub 2022 Feb 16.
2
Molecular and biological functions of TRIM-NHL RNA-binding proteins.TRIM-NHL RNA 结合蛋白的分子和生物学功能。
Wiley Interdiscip Rev RNA. 2021 Mar;12(2):e1620. doi: 10.1002/wrna.1620. Epub 2020 Aug 1.
3
GLP-1 Notch-LAG-1 CSL control of the germline stem cell fate is mediated by transcriptional targets lst-1 and sygl-1.
COP9信号体组分CSN-5在秀丽隐杆线虫生殖系干细胞和祖细胞中稳定PUF蛋白FBF-1和FBF-2。
Genetics. 2024 May 7;227(1). doi: 10.1093/genetics/iyae033.
4
DOT-1.1 (DOT1L) deficiency in leads to small RNA-dependent gene activation.DOT-1.1（DOT1L）缺陷导致小RNA依赖的基因激活。
BBA Adv. 2023 Feb 1;3:100080. doi: 10.1016/j.bbadva.2023.100080. eCollection 2023.
5
The role of RNA-binding proteins in orchestrating germline development in .RNA结合蛋白在调控……种系发育中的作用
Front Cell Dev Biol. 2023 Jan 4;10:1094295. doi: 10.3389/fcell.2022.1094295. eCollection 2022.
GLP-1 Notch-LAG-1 CSL 通过转录靶标 lst-1 和 sygl-1 来控制生殖干细胞命运。
PLoS Genet. 2020 Mar 20;16(3):e1008650. doi: 10.1371/journal.pgen.1008650. eCollection 2020 Mar.
4
Biology of the Germline Stem Cell System.生殖干细胞系统生物学。
Genetics. 2019 Dec;213(4):1145-1188. doi: 10.1534/genetics.119.300238.
5
A PUF Hub Drives Self-Renewal in Germline Stem Cells.PUF 集线器驱动生殖干细胞自我更新。
Genetics. 2020 Jan;214(1):147-161. doi: 10.1534/genetics.119.302772. Epub 2019 Nov 18.
6
Identification of proteins and miRNAs that specifically bind an mRNA in vivo.在体内鉴定与特定 mRNA 结合的蛋白质和 miRNA。
Nat Commun. 2019 Sep 16;10(1):4205. doi: 10.1038/s41467-019-12050-7.
7
The molecular basis of LST-1 self-renewal activity and its control of stem cell pool size.LST-1 自我更新活性的分子基础及其对干细胞池大小的控制。
Development. 2019 Oct 17;146(20):dev181644. doi: 10.1242/dev.181644.
8
Rapid population-wide declines in stem cell number and activity during reproductive aging in .生殖衰老过程中， 大量干细胞数量和活性的迅速下降。
Development. 2019 Apr 23;146(8):dev173195. doi: 10.1242/dev.173195.
9
The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in . e.TRIM-NHL 蛋白 NHL-2 是. e. 中核和体细胞 RNAi 途径的辅助因子。
Elife. 2018 Dec 21;7:e35478. doi: 10.7554/eLife.35478.
10
Functional genomic analysis identifies miRNA repertoire regulating C. elegans oocyte development.功能基因组分析鉴定调控秀丽隐杆线虫卵母细胞发育的 miRNA 组。
Nat Commun. 2018 Dec 14;9(1):5318. doi: 10.1038/s41467-018-07791-w.