Laboratory of Renal Lithiasis Research, University Institute of Health Sciences Research (IUNICS-IdISBa), University of Balearic Islands, 07122, Palma, Spain.
Laboratory of Infection and Immunity, University Institute of Health Sciences Research (IUNICS-IdISBa), University of Balearic Islands, 07122, Palma, Spain.
Urolithiasis. 2022 Dec;50(6):737-742. doi: 10.1007/s00240-022-01350-1. Epub 2022 Sep 5.
The use of double J ureteral stents can lead to several adverse effects, as urinary infection. Bacteria tend to colonize the stent surface, leading to the formation of bacterial biofilms. The presence of urease-producing bacteria increase the urine pH leading to the incrustation and blockage of the stent. On the other hand, these large crystalline masses function as niduses, allowing the attachment of even more bacteria and decreasing its exposure to antibiotics. The aim of this in vitro study was to assess the effect of phytate on the attachment of bacteria to the catheter surface under conditions that favor crystallization. Catheter sections were incubated in a synthetic urine medium (pH 6.5) in the presence or absence of Pseudomonas aeruginosa and phytate. Amount of calcium deposits was measured using an Arsenazo III colorimetric method and the number of attached bacteria to the stent was determined. Differences were assessed using an ANOVA with a Bonferroni post hoc test. The formation of calcium phosphate deposits (brushite and hydroxyapatite) and oxalate crystals (COM), as were as the amount of bacteria decreased when phytate was present. Thus, phytate successfully decreased bacterial adhesion by inhibiting the formation of crystalline deposits.
双 J 输尿管支架的使用可能会导致多种不良反应，如尿路感染。细菌往往会在支架表面定植，导致细菌生物膜的形成。产脲酶细菌的存在会增加尿液 pH 值，导致支架上的结石和堵塞。另一方面，这些大的结晶团块作为巢穴，允许更多的细菌附着，并减少其对抗生素的暴露。本体外研究的目的是评估植酸在有利于结晶形成的条件下，对细菌附着到导管表面的影响。在含有或不含有铜绿假单胞菌和植酸的合成尿液培养基中孵育导管段。使用 Arsenazo III 比色法测量钙沉积物的量，并确定附着在支架上的细菌数量。使用方差分析和 Bonferroni 事后检验评估差异。当植酸存在时，会形成磷酸钙沉积物（鸟粪石和羟磷灰石）和草酸盐晶体（COM），以及细菌数量减少。因此，植酸通过抑制结晶沉积物的形成成功地减少了细菌的黏附。
