正常、糖尿病及胰高血糖素处理大鼠肝脏线粒体中肉碱棕榈酰转移酶I的体外激活研究。
Studies on the activation in vitro of carnitine palmitoyltransferase I in liver mitochondria from normal, diabetic and glucagon-treated rats.
作者信息
Grantham B D, Zammit V A
出版信息
Biochem J. 1987 Apr 1;243(1):261-5. doi: 10.1042/bj2430261.
Abstract
The activation of overt carnitine palmitoyltransferase activity that occurs when rat liver mitochondria are incubated at near-physiological temperatures and ionic strengths was studied for mitochondria obtained from animals in different physiological states. In all instances, it was found to be due exclusively to an increase in the catalytic capacity of the enzyme and not to an increase in affinity of the enzyme for palmitoyl-CoA. The enzyme in mitochondria from fed animals always showed a larger degree of activation than that in mitochondria from starved animals. This was the case even for mitochondria (e.g. from fed diabetic animals) in which the kinetic characteristics of carnitine palmitoyltransferase were more similar to those for the enzyme in mitochondria from starved rats. Glucagon treatment of rats before isolation of the mitochondria did not affect the characteristics either of the kinetic parameters of overt carnitine palmitoyltransferase or of its activation in vitro.
摘要
对处于不同生理状态的动物的线粒体进行研究，观察当大鼠肝脏线粒体在接近生理温度和离子强度下孵育时，明显的肉碱棕榈酰转移酶活性的激活情况。在所有情况下，发现这完全是由于该酶催化能力的增加，而不是由于该酶对棕榈酰辅酶A亲和力的增加。喂食动物的线粒体中的酶总是比饥饿动物的线粒体中的酶表现出更大程度的激活。即使对于肉碱棕榈酰转移酶的动力学特性与饥饿大鼠线粒体中的酶更相似的线粒体（例如来自喂食糖尿病动物的线粒体）也是如此。在分离线粒体之前用胰高血糖素处理大鼠，既不影响明显的肉碱棕榈酰转移酶动力学参数的特征，也不影响其体外激活。
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Studies on the activation in vitro of carnitine palmitoyltransferase I in liver mitochondria from normal, diabetic and glucagon-treated rats.正常、糖尿病及胰高血糖素处理大鼠肝脏线粒体中肉碱棕榈酰转移酶I的体外激活研究。
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本文引用的文献
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