在体外将完整大鼠肝脏线粒体与微摩尔浓度的丙二酰辅酶A预孵育后，肉碱棕榈酰转移酶I活性对丙二酰辅酶A抑制作用的敏感性增加。
Increased sensitivity of carnitine palmitoyltransferase I activity to malonyl-CoA inhibition after preincubation of intact rat liver mitochondria with micromolar concentrations of malonyl-CoA in vitro.
作者信息
Zammit V A
出版信息
Biochem J. 1983 Mar 15;210(3):953-6. doi: 10.1042/bj2100953.
Abstract
Carnitine palmitoyltransferase I in rat liver mitochondria preincubated with malonyl-CoA was more sensitive to inhibition by malonyl-CoA than was the enzyme in mitochondria preincubated in the absence of malonyl-CoA. For carnitine palmitoyltransferase I in mitochondria from starved animals this increase also resulted in the enzyme becoming significantly more sensitive than that in mitochondria assayed immediately after their isolation. Concentrations of malonyl-CoA that induced half the maximal degree of sensitization observed were 1-3 microM.
摘要
与在无丙二酸单酰辅酶A的情况下预孵育的线粒体中的酶相比，在与丙二酸单酰辅酶A预孵育的大鼠肝脏线粒体中，肉碱棕榈酰转移酶I 对丙二酸单酰辅酶A的抑制作用更敏感。对于饥饿动物线粒体中的肉碱棕榈酰转移酶I，这种增加还导致该酶比分离后立即测定的线粒体中的酶明显更敏感。诱导观察到的最大致敏程度一半的丙二酸单酰辅酶A浓度为1-3微摩尔。
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Increased sensitivity of carnitine palmitoyltransferase I activity to malonyl-CoA inhibition after preincubation of intact rat liver mitochondria with micromolar concentrations of malonyl-CoA in vitro.在体外将完整大鼠肝脏线粒体与微摩尔浓度的丙二酰辅酶A预孵育后，肉碱棕榈酰转移酶I活性对丙二酰辅酶A抑制作用的敏感性增加。
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本文引用的文献
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Importance of experimental conditions in evaluating the malonyl-CoA sensitivity of liver carnitine acyltransferase. Studies with fed and starved rats.实验条件在评估肝脏肉碱酰基转移酶丙二酸单酰辅酶A敏感性中的重要性。对喂食和饥饿大鼠的研究。
Biochem J. 1981 Nov 15;200(2):217-23. doi: 10.1042/bj2000217.
2
Regulation of hepatic fatty acid metabolism. The activities of mitochondrial and microsomal acyl-CoA:sn-glycerol 3-phosphate O-acyltransferase and the concentrations of malonyl-CoA, non-esterified and esterified carnitine, glycerol 3-phosphate, ketone bodies and long-chain acyl-CoA esters in livers of fed or starved pregnant, lactating and weaned rats.肝脏脂肪酸代谢的调节。喂食或饥饿的怀孕、哺乳和断奶大鼠肝脏中线粒体和微粒体酰基辅酶A：sn-甘油3-磷酸O-酰基转移酶的活性以及丙二酰辅酶A、非酯化和酯化肉碱、甘油3-磷酸、酮体和长链酰基辅酶A酯的浓度。
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