在体外将完整大鼠肝脏线粒体与微摩尔浓度的丙二酰辅酶A预孵育后，肉碱棕榈酰转移酶I活性对丙二酰辅酶A抑制作用的敏感性增加。
Increased sensitivity of carnitine palmitoyltransferase I activity to malonyl-CoA inhibition after preincubation of intact rat liver mitochondria with micromolar concentrations of malonyl-CoA in vitro.
作者信息
Zammit V A
出版信息
Biochem J. 1983 Mar 15;210(3):953-6. doi: 10.1042/bj2100953.
Abstract
Carnitine palmitoyltransferase I in rat liver mitochondria preincubated with malonyl-CoA was more sensitive to inhibition by malonyl-CoA than was the enzyme in mitochondria preincubated in the absence of malonyl-CoA. For carnitine palmitoyltransferase I in mitochondria from starved animals this increase also resulted in the enzyme becoming significantly more sensitive than that in mitochondria assayed immediately after their isolation. Concentrations of malonyl-CoA that induced half the maximal degree of sensitization observed were 1-3 microM.
摘要
与在无丙二酸单酰辅酶A的情况下预孵育的线粒体中的酶相比，在与丙二酸单酰辅酶A预孵育的大鼠肝脏线粒体中，肉碱棕榈酰转移酶I 对丙二酸单酰辅酶A的抑制作用更敏感。对于饥饿动物线粒体中的肉碱棕榈酰转移酶I，这种增加还导致该酶比分离后立即测定的线粒体中的酶明显更敏感。诱导观察到的最大致敏程度一半的丙二酸单酰辅酶A浓度为1-3微摩尔。
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本文引用的文献
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