在分离的大鼠肝脏线粒体中，肉碱酰基转移酶I对丙二酰辅酶A抑制作用的敏感性与体内肝脏丙二酰辅酶A浓度存在定量关系。
Sensitivity of carnitine acyltransferase I to malonly-CoA inhibition in isolated rat liver mitochondria is quantitatively related to hepatic malonyl-CoA concentration in vivo.
作者信息
Robinson I N, Zammit V A
出版信息
Biochem J. 1982 Jul 15;206(1):177-9. doi: 10.1042/bj2060177.
Abstract
The sensitivity of carnitine acyltransferase I (EC 2.3.1.21) activity to malonyl-CoA inhibition in rat liver mitochondria isolated from animals in various physiological states was quantitatively proportional to the hepatic malonyl-CoA concentration in vivo. It is suggested that this relationship between the two parameters could result in a potent amplification mechanism for the reciprocal regulation of fatty acid synthesis and oxidation.
摘要
从处于不同生理状态的动物分离得到的大鼠肝脏线粒体中，肉碱脂酰转移酶I（EC 2.3.1.21）活性对丙二酰辅酶A抑制作用的敏感性与体内肝脏丙二酰辅酶A浓度呈定量比例关系。有人提出，这两个参数之间的这种关系可能导致脂肪酸合成与氧化相互调节的有效放大机制。
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Sensitivity of carnitine acyltransferase I to malonly-CoA inhibition in isolated rat liver mitochondria is quantitatively related to hepatic malonyl-CoA concentration in vivo.在分离的大鼠肝脏线粒体中，肉碱酰基转移酶I对丙二酰辅酶A抑制作用的敏感性与体内肝脏丙二酰辅酶A浓度存在定量关系。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The effect of glucagon treatment and starvation of virgin and lactating rats on the rates of oxidation of octanoyl-L-carnitine and octanoate by isolated liver mitochondria.胰高血糖素处理以及处女鼠和泌乳期大鼠饥饿对离体肝线粒体中辛酰-L-肉碱和辛酸氧化速率的影响。
Biochem J. 1980 Aug 15;190(2):293-300. doi: 10.1042/bj1900293.
3
Importance of experimental conditions in evaluating the malonyl-CoA sensitivity of liver carnitine acyltransferase. Studies with fed and starved rats.实验条件在评估肝脏肉碱酰基转移酶丙二酸单酰辅酶A敏感性中的重要性。对喂食和饥饿大鼠的研究。
Biochem J. 1981 Nov 15;200(2):217-23. doi: 10.1042/bj2000217.
4
Regulation of hepatic fatty acid metabolism. The activities of mitochondrial and microsomal acyl-CoA:sn-glycerol 3-phosphate O-acyltransferase and the concentrations of malonyl-CoA, non-esterified and esterified carnitine, glycerol 3-phosphate, ketone bodies and long-chain acyl-CoA esters in livers of fed or starved pregnant, lactating and weaned rats.肝脏脂肪酸代谢的调节。喂食或饥饿的怀孕、哺乳和断奶大鼠肝脏中线粒体和微粒体酰基辅酶A：sn-甘油3-磷酸O-酰基转移酶的活性以及丙二酰辅酶A、非酯化和酯化肉碱、甘油3-磷酸、酮体和长链酰基辅酶A酯的浓度。
Biochem J. 1981 Jul 15;198(1):75-83. doi: 10.1042/bj1980075.
5
Evaluation of malonyl-CoA in the regulation of long-chain fatty acid oxidation in the liver. Evidence for an unidentified regulatory component of the system.肝脏中丙二酰辅酶A对长链脂肪酸氧化调节作用的评估。该系统存在未知调节成分的证据。
Biochem J. 1980 Dec 15;192(3):959-62. doi: 10.1042/bj1920959.
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7
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10
Characteristics of fatty acid oxidation in rat liver homogenates and the inhibitory effect of malonyl-CoA.
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