通过 PNA FISH 探针检测端粒异常。

Telomere Aberration Detection by PNA FISH Probe.

机构信息

Department of Environmental & Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA.

出版信息

Methods Mol Biol. 2023;2519:105-110. doi: 10.1007/978-1-0716-2433-3_12.

Abstract

Telomere is a structure of the end cap of chromosomes. Telomere gets shorter as cell aging and progressing cell division. Shorter telomere may cause telomere fusion, thus inducing genomic instability. Telomere dysfunction can be visualized by PNA FISH probe against telomere repeat sequence (TTAGGG)n. PNA probes have higher hybridization affinity than DNA probes. The traditional FISH or modified FISH protocol can stain telomere relatively easier than whole-chromosome painting probes. This chapter introduces PNA telomere FISH protocol to visualize telomere signals on metaphase chromosomes.

摘要

端粒是染色体末端的一种结构。随着细胞衰老和细胞分裂的进行，端粒会变短。较短的端粒可能导致端粒融合，从而导致基因组不稳定。端粒功能障碍可以通过针对端粒重复序列（TTAGGG）n 的 PNA FISH 探针来可视化。PNA 探针比 DNA 探针具有更高的杂交亲和力。传统的 FISH 或改良的 FISH 方案比整条染色体涂染探针更容易对端粒进行染色。本章介绍 PNA 端粒 FISH 方案，以显示中期染色体上的端粒信号。