School of Materials Science and Engineering and Key Laboratory of Biomedical Materials of Ministry of Education, 26467South China University of Technology, Guangzhou, China.
National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China.
J Biomater Appl. 2023 Jan;37(6):1007-1017. doi: 10.1177/08853282221124367. Epub 2022 Sep 6.
Biphasic calcium phosphate (BCP) scaffold has been widely applied to bone regeneration because of its good biocompatibility and bone conduction property. However, the low mechanical strength and the lack of angiogenic and osteogenic induction properties have restricted its application in bone tissue regeneration. In this study, we combined zinc (Zn) doping and silk fibroin (SF) coating with expectation to enhance compressive strength, osteogenesis and angiogenesis of BCP scaffolds. The phase composition, morphology, porosity, compressive strength, degradation and cell behaviors were investigated systematically. Results showed that the scaffold coated with SF exhibited almost 3 times of compressive strength without compromising its porosity compared with the uncoated scaffold. Zn doping and SF coating synergistically enhanced the alkaline phosphatase activity and osteogenesis-related genes expression of mouse bone mesenchymal stem cells (mBMSCs). Furthermore, SF coating notably improved the proliferation, cell viability and angiogenesis of human umbilical vein endothelial cells (HUVECs). This work provides a novel way to modify BCP scaffolds simultaneously with enhancing mechanical strength and biological properties.
双相磷酸钙（BCP）支架由于其良好的生物相容性和骨传导性而被广泛应用于骨再生。然而，其低机械强度和缺乏血管生成和成骨诱导特性限制了其在骨组织再生中的应用。在这项研究中，我们将锌（Zn）掺杂和丝素蛋白（SF）涂层相结合，期望提高 BCP 支架的抗压强度、成骨和血管生成能力。系统研究了支架的相组成、形貌、孔隙率、抗压强度、降解和细胞行为。结果表明，与未涂层支架相比，SF 涂层后的支架的抗压强度几乎提高了 3 倍，而孔隙率没有降低。Zn 掺杂和 SF 涂层协同增强了小鼠骨髓间充质干细胞（mBMSCs）的碱性磷酸酶活性和成骨相关基因表达。此外，SF 涂层显著提高了人脐静脉内皮细胞（HUVECs）的增殖、细胞活力和血管生成。这项工作为同时提高机械强度和生物性能来修饰 BCP 支架提供了一种新方法。
