Lu Teliang, Li Guohao, Zhang Luhui, Yuan Xinyuan, Wu Tingting, Ye Jiandong
School of Materials Science and Engineering and Key Laboratory of Biomedical Materials of Ministry of Education, South China University of Technology, Guangzhou, 510641, PR China.
National Engineering Research Center for Healthcare Devices, Guangdong Key Lab of Medical Electronic Instruments and Polymer Material Products, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou, Guangdong, 510316, PR China.
Mater Today Bio. 2024 Aug 14;28:101203. doi: 10.1016/j.mtbio.2024.101203. eCollection 2024 Oct.
Biphasic calcium phosphate (BCP) ceramics are valued for their osteoconductive properties but have limited osteogenic and angiogenic activities, which restricts their clinical utility in bone defect repair. Silicon doping has emerged as an effective strategy to enhance these biological functions of BCP. However, the biological impact of BCP is influenced by the level of silicon doping, necessitating determination of the optimal concentration to maximize efficacy in bone repair. This study investigated the effects of silicon doping on both the physicochemical and biological properties of BCP, with a specific focus on osteogenic and angiogenic potentials. Results indicated that silicon doping exceeding 4 mol.% led to the formation of α-TCP, accelerating BCP degradation, enhancing silicon ion release, and promoting mineralization product formation. Simultaneously, silicon doping increased the porosity of BCP scaffolds, which typically reduces their compressive strength. Nevertheless, scaffolds doped with ≤4 mol.% silicon maintained compressive strengths exceeding 2 MPa. biological experiments indicated that higher levels of silicon doping (≥6 mol.%) partially inhibited the successful differentiation of stem cells and the vascularization of endothelial cells. Optimal conditions for promoting osteogenic differentiation and angiogenesis were identified between 2 and 4 mol.% silicon doping, with an optimal level of approximately 4 mol.%. Subsequent experiments confirmed that BCP scaffolds doped with 4 mol.% silicon effectively promoted vascularization and new bone formation, highlighting their potential for clinical bone defect repair.
双相磷酸钙（BCP）陶瓷因其骨传导特性而受到重视，但其成骨和血管生成活性有限，这限制了它们在骨缺损修复中的临床应用。硅掺杂已成为增强BCP这些生物学功能的有效策略。然而，BCP的生物学影响受硅掺杂水平的影响，因此需要确定最佳浓度以最大化骨修复效果。本研究调查了硅掺杂对BCP物理化学和生物学特性的影响，特别关注其成骨和血管生成潜力。结果表明，硅掺杂超过4摩尔％会导致α-TCP的形成，加速BCP降解，增强硅离子释放，并促进矿化产物形成。同时，硅掺杂增加了BCP支架的孔隙率，这通常会降低其抗压强度。然而，掺杂≤4摩尔％硅的支架抗压强度仍超过2MPa。生物学实验表明，较高水平的硅掺杂（≥6摩尔％）会部分抑制干细胞的成功分化和内皮细胞的血管生成。在2至4摩尔％的硅掺杂之间确定了促进成骨分化和血管生成的最佳条件，最佳水平约为4摩尔％。随后的实验证实，掺杂4摩尔％硅的BCP支架有效地促进了血管生成和新骨形成，突出了它们在临床骨缺损修复中的潜力。
