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双层磷酸钙支架的多层丝素涂层：对物理力学性能和人骨髓间充质干细胞体外成骨反应的影响。

Multiple silk coatings on biphasic calcium phosphate scaffolds: effect on physical and mechanical properties and in vitro osteogenic response of human mesenchymal stem cells.

机构信息

Department of Biomedical Engineering, Tufts University, Medford, Massachusetts 02155, United States.

出版信息

Biomacromolecules. 2013 Jul 8;14(7):2179-88. doi: 10.1021/bm400303w. Epub 2013 Jun 7.

DOI:10.1021/bm400303w
PMID:23745709
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3752092/
Abstract

Ceramic scaffolds such as biphasic calcium phosphate (BCP) have been widely studied and used for bone regeneration, but their brittleness and low mechanical strength are major drawbacks. We report the first systematic study on the effect of silk coating in improving the mechanical and biological properties of BCP scaffolds, including (1) optimization of the silk coating process by investigating multiple coatings, and (2) in vitro evaluation of the osteogenic response of human mesenchymal stem cells (hMSCs) on the coated scaffolds. Our results show that multiple silk coatings on BCP ceramic scaffolds can achieve a significant coating effect to approach the mechanical properties of native bone tissue and positively influence osteogenesis by hMSCs over an extended period. The silk coating method developed in this study represents a simple yet effective means of reinforcement that can be applied to other types of ceramic scaffolds with similar microstructure to improve osteogenic outcomes.

摘要

陶瓷支架（如双相磷酸钙，BCP）已被广泛研究和用于骨再生，但它们的脆性和低机械强度是主要缺点。我们报告了第一个关于丝素涂层在改善 BCP 支架的机械和生物学性能方面的系统研究，包括（1）通过研究多种涂层优化丝素涂层工艺，以及（2）在体外评估涂层支架上人骨髓间充质干细胞（hMSC）的成骨反应。我们的结果表明，BCP 陶瓷支架上的多次丝素涂层可以实现显著的涂层效果，接近天然骨组织的机械性能，并通过 hMSC 延长时间积极影响成骨。本研究中开发的丝素涂层方法代表了一种简单而有效的增强方法，可应用于具有类似微观结构的其他类型的陶瓷支架，以改善成骨效果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f82f/3752092/0a4ba784e850/nihms490884f9.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f82f/3752092/7f851c7248f8/nihms490884f5.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f82f/3752092/624c85c93e66/nihms490884f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f82f/3752092/0a4ba784e850/nihms490884f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f82f/3752092/7e1d5b191ac5/nihms490884f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f82f/3752092/9e2460113173/nihms490884f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f82f/3752092/7f851c7248f8/nihms490884f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f82f/3752092/3fc6bbcf0536/nihms490884f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f82f/3752092/2af649270fe2/nihms490884f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f82f/3752092/624c85c93e66/nihms490884f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f82f/3752092/0a4ba784e850/nihms490884f9.jpg

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1
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2
Substituted hydroxyapatites for bone repair.用于骨修复的羟基磷灰石替代物。
J Mater Sci Mater Med. 2012 Oct;23(10):2335-47. doi: 10.1007/s10856-012-4598-2. Epub 2012 Mar 3.
3
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Bioact Mater. 2021 Dec 25;15:82-102. doi: 10.1016/j.bioactmat.2021.12.005. eCollection 2022 Sep.
4
Natural and Synthetic Polymers for Bone Scaffolds Optimization.用于骨支架优化的天然和合成聚合物
Polymers (Basel). 2020 Apr 14;12(4):905. doi: 10.3390/polym12040905.
5
Application of a novel porous tantalum implant in rabbit anterior lumbar spine fusion model: in vitro and in vivo experiments.新型多孔钽植入物在兔前路腰椎融合模型中的应用：体外与体内实验。
Chin Med J (Engl). 2019 Jan 5;132(1):51-62. doi: 10.1097/CM9.0000000000000030.
6
3D bioactive composite scaffolds for bone tissue engineering.用于骨组织工程的3D生物活性复合支架
Bioact Mater. 2017 Dec 1;3(3):278-314. doi: 10.1016/j.bioactmat.2017.10.001. eCollection 2018 Sep.
7
Silk fibroin/hydroxyapatite composite hydrogel induced by gamma-ray irradiation for bone tissue engineering.用于骨组织工程的γ射线辐照诱导丝素蛋白/羟基磷灰石复合水凝胶
Biomater Res. 2017 Jun 24;21:12. doi: 10.1186/s40824-017-0098-2. eCollection 2017.
8
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9
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10
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5
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J Biomed Mater Res A. 2011 Oct;99(1):16-28. doi: 10.1002/jbm.a.33158. Epub 2011 Jul 25.
6
Bone regeneration: current concepts and future directions.骨再生：当前概念与未来方向。
BMC Med. 2011 May 31;9:66. doi: 10.1186/1741-7015-9-66.
7
Effects of bioactive glass nanoparticles on the mechanical and biological behavior of composite coated scaffolds.生物活性玻璃纳米粒子对复合涂层支架的力学和生物学行为的影响。
Acta Biomater. 2011 Mar;7(3):1307-18. doi: 10.1016/j.actbio.2010.10.015. Epub 2010 Nov 10.
8
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9
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10
The influence hydroxyapatite nanoparticle shape and size on the properties of biphasic calcium phosphate scaffolds coated with hydroxyapatite-PCL composites.羟基磷灰石纳米颗粒的形状和尺寸对羟基磷灰石-PCL 复合材料涂层双相磷酸钙支架性能的影响。
Biomaterials. 2010 Jul;31(21):5498-509. doi: 10.1016/j.biomaterials.2010.03.058. Epub 2010 Apr 15.