初级 microRNA 加工中的动态蛋白-RNA 识别。
Dynamic Protein-RNA recognition in primary MicroRNA processing.
机构信息
Department of Biochemistry, Department of Biophysics, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: https://twitter.com/@Ruiz_Arroy0.
Department of Biochemistry, Department of Biophysics, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
出版信息
Curr Opin Struct Biol. 2022 Oct;76:102442. doi: 10.1016/j.sbi.2022.102442. Epub 2022 Sep 5.
MicroRNAs are prevalent regulators of gene expression, controlling most of the proteome in multicellular organisms. To generate the functional small RNAs, precise processing steps are required. In animals, microRNA biogenesis is initiated by Microprocessor that minimally consists of the Drosha enzyme and its partner, DGCR8. This first step is critical for selecting primary microRNAs, and many RNA-binding proteins and regulatory pathways target both the accuracy and efficiency of microRNA maturation. Structures of Drosha and DGCR8 in complex with primary microRNAs elucidate how RNA structural features rather than sequence provide the framework for substrate recognition. Comparing multiple states of Microprocessor and the closely related Dicer homologs shed light on the dynamic protein-RNA complex assembly and disassembly required to recognize RNAs with diverse sequences via common structural features.
微 RNA 是基因表达的普遍调控因子，控制着多细胞生物中的大多数蛋白质组。为了生成功能性的小 RNA，需要精确的加工步骤。在动物中，微 RNA 的生物发生是由 Microprocessor 启动的，它至少由 Drosha 酶及其伴侣 DGCR8 组成。这第一步对于选择初级微 RNA 至关重要，许多 RNA 结合蛋白和调节途径都针对微 RNA 成熟的准确性和效率。Drosha 和 DGCR8 与初级微 RNA 形成复合物的结构阐明了 RNA 结构特征而不是序列如何为底物识别提供框架。比较 Microprocessor 的多种状态和密切相关的 Dicer 同源物，揭示了通过常见的结构特征识别具有不同序列的 RNA 所需的动态蛋白-RNA 复合物组装和拆卸。
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