Phoonsawat Kamonchanok, Agir Ismail, Dungchai Wijitar, Ozer Tugba, Henry Charles S
Organic Synthesis, Electrochemistry & Natural Product Research Unit, Department of Chemistry, Faculty of Science, King Mongkut's University of Technology Thonburi, Prachautid Road, Thungkru, Bangkok, 10140, Thailand; Department of Chemistry, Colorado State University, Fort Collins, CO, 80523, United States.
Department of Bioengineering, Faculty of Engineering and Natural Sciences, Istanbul Medeniyet University, Istanbul, Turkey.
Anal Chim Acta. 2022 Sep 15;1226:340245. doi: 10.1016/j.aca.2022.340245. Epub 2022 Aug 19.
At home electrolyte analysis is of growing interest due to the importance for early diagnosis for various diseases. This work presents the first device that integrates a potentiometric ion-selective electrode (ISE) with distance-based colorimetric detection for the simultaneous analysis of K and Cl ions at the point of care (POC). This hybrid sensing device was designed in a two-dimensional configuration using a plastic transparency sheet containing a stencil printed K-ISE and a paper substrate for distance-based colorimetric detection of Cl. K quantification was performed using a low-cost (<$25) lab-made Wi-Fi supported potentiometer with a custom smartphone application while Cl ions were quantified with an instrument-free distance-based paper analytical device (dPAD). The total analysis time is 5 min once the sample is added. The K-ISE showed a sensitivity of 55.89 ± 2.52 mV/decade from 0.1 to 100 mM with a limit of detection (LOD) of 0.01 mM. The linear working range for Cl detection was 0.5-50 mM whereas the LOD was 0.16 ± 0.05 mM. The proof-of-concept application of the sensing hybrid device was demonstrated in human urine and artificial sweat samples containing K and Cl ions at physiologically relevant ranges. The recoveries were found to be 88-108% for K and 90-104% for Cl, showing the potential use of the proposed device for low-cost routine analysis of biological samples at POC.
由于对各种疾病早期诊断的重要性，家庭电解质分析越来越受到关注。这项工作展示了首个将电位离子选择性电极（ISE）与基于距离的比色检测相结合的装置，用于在护理点（POC）同时分析钾离子和氯离子。这种混合传感装置采用二维结构设计，使用含有模板印刷钾离子选择性电极的塑料透明片和用于氯离子基于距离比色检测的纸质基底。使用低成本（<25美元）的实验室自制Wi-Fi支持电位计和定制智能手机应用程序进行钾离子定量，而氯离子则通过无仪器的基于距离的纸质分析装置（dPAD）进行定量。加入样品后，总分析时间为5分钟。钾离子选择性电极在0.1至100 mM范围内的灵敏度为55.89±2.52 mV/十倍浓度变化，检测限（LOD）为0.01 mM。氯离子检测的线性工作范围为0.5 - 50 mM，而检测限为0.16±0.05 mM。在含有生理相关范围内钾离子和氯离子的人类尿液和人工汗液样本中展示了传感混合装置的概念验证应用。钾离子的回收率为88 - 108%，氯离子的回收率为90 - 104%，表明所提出的装置在护理点对生物样品进行低成本常规分析具有潜在用途。
